Literature summary for 2.4.1.207 extracted from

Johansson, P.; Brumer, H.3rd.; Baumann, M.J.; Kallas, A.M.; Henriksson, H.; Denman, S.E.; Teeri, T.T.; Jones, T.A.
Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding (2004), Plant Cell, 16, 874-886.

Search for more literature for 2.4.1.207

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, functional expression in Pichia pastoris	Populus tremula x Populus tremuloides

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, free enzyme or in complex with a xyloglucan nonasaccharide XLLG, hanging drop vapour diffusion method, 20°C, 0.002 ml of 10 mg/ml protein in 10 mM HEPES, pH 7.0, mixed with equal volume of a solution containing 1.0 M NaOAc and 0.2 M imidazole, and again mixed with equal volume of reservoir solution containing 0.4 M potassium sodium tartrate, X-ray diffraction structure determination and analysis at 1.8 A resolution, overall structure is a beta-sandwich, molecular modeling	Populus tremula x Populus tremuloides

Crystallization (Comment)

Organism

purified recombinant enzyme, free enzyme or in complex with a xyloglucan nonasaccharide XLLG, hanging drop vapour diffusion method, 20°C, 0.002 ml of 10 mg/ml protein in 10 mM HEPES, pH 7.0, mixed with equal volume of a solution containing 1.0 M NaOAc and 0.2 M imidazole, and again mixed with equal volume of reservoir solution containing 0.4 M potassium sodium tartrate, X-ray diffraction structure determination and analysis at 1.8 A resolution, overall structure is a beta-sandwich, molecular modeling

Populus tremula x Populus tremuloides

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Populus tremula x Populus tremuloides	5618	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Populus tremula x Populus tremuloides	key enzyme in all plant processes that require cell wall remodeling	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Populus tremula x Populus tremuloides	Q8GZD5	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Pichia pastoris by strong cation exchange chromatography and gel filtration in a sequential combination	Populus tremula x Populus tremuloides

Reaction

Reaction	Comment	Organism	Reaction ID
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc	substrate binding and catalytic mechanism, active site structure, structure-function study	Populus tremula x Populus tremuloides	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	key enzyme in all plant processes that require cell wall remodeling	Populus tremula x Populus tremuloides	?	-	?
additional information	transglycosylation acceptor binding mechanism, structure-function study	Populus tremula x Populus tremuloides	?	-	?
xyloglucan nonasaccharide + xyloglucan	nonasaccharide XLLG, a 2-chloro-4-nitrophenyl-nonasaccharide is no substrate, tamarind xyloglucan	Populus tremula x Populus tremuloides	?	-	?

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16	Populus tremula x Populus tremuloides
XET16A	-	Populus tremula x Populus tremuloides
xyloglucan endotransglucosylase 16A	-	Populus tremula x Populus tremuloides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Populus tremula x Populus tremuloides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.6	-	assay at	Populus tremula x Populus tremuloides