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Literature summary for 2.4.1.207 extracted from
- Differences in catalytic properties between native isoenzymes of xyloglucan endotransglycosylase (XET) (2000), Phytochemistry, 54, 667-680.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme M55a | Vigna radiata var. radiata |  |
| 0.028 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme M45 | Vigna radiata var. radiata | |
| 0.031 | 0.035 | Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzymes M35a and M35b | Vigna radiata var. radiata | |
| 0.032 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme M55b | Vigna radiata var. radiata | |
| 0.071 | 0.082 | Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzymes C45a and C45b | Brassica oleracea | |
| 0.13 | - |
Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzyme C30 | Brassica oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, each isoenzyme, SDS-PAGE | Vigna radiata var. radiata |
| 32000 | - |
x * 32000, each isoenzyme, SDS-PAGE | Brassica oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + xyloglucan acceptor | Vigna radiata var. radiata | existence of different classes of XET with differing roles in vivo | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | Brassica oleracea | existence of different classes of XET with differing roles in vivo | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | Brassica oleracea | major role is the integration of new xyloglucan into the cell walls of the densely cytoplasmic florets | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | Vigna radiata var. radiata | associated with primary cell wall metabolism, rather than mobilisation of any seed storage xyloglucan, major role is the re-structuring of existing wall material in the rapidly vacuolating shoots | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
cauliflower | - |
| Brassica oleracea | - |
var. botrytis, 2 isoenzymes: C30, C45 | - |
| Vigna radiata var. radiata | - |
mung bean | - |
| Vigna radiata var. radiata | - |
Vigna radiata | - |
| Vigna radiata var. radiata | - |
4 isoenzymes: M35, M45, M55a, M55b | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata var. radiata |
- |
Brassica oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| floret | - |
Brassica oleracea | - |
| seedling | sprouting seedlings, more abundant in the growing tissues of the hypocotyls and leaves than in the cotyledons | Vigna radiata var. radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| donor xyloglucan + acceptor xyloglucan | acceptor specificity | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | acceptor specificity | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | acceptors: polymer xyloglucan or its derived oligosaccharides | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | pronounced preference for xyloglucan oligosaccharides with backbones of 4 over 3 over 2 Glc residues as acceptors | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | pronounced preference for xyloglucan oligosaccharides with backbones of 4 over 3 over 2 Glc residues as acceptors | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | xyloglucan from Tamarindus indica | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + acceptor xyloglucan | xyloglucan from Tamarindus indica | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc-ol | pentamer XXG-ol, all isoenzymes have low but significant activity, tamarind xyloglucan as donor | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)(Xyl(1-6))Glc(1-4)Glc-ol | pentamer XXG-ol, all isoenzymes have low but significant activity, tamarind xyloglucan as donor | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xyl(1-6)Glc(1-4)Glc | trimer XG-ol, only isomer M55a shows a slight activity, tamarind xyloglucan as donor | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | 1.7-3.6fold preference of XLLG-ol over XXXG-ol, mung bean isoenzymes have higher affinities for XLLG-ol than cauliflower isoenzymes | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | 1.7-3.6fold preference of XLLG-ol over XXXG-ol, mung bean isoenzymes have higher affinities for XLLG-ol than cauliflower isoenzymes | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | donor: tamarind xyloglucan | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | donor: tamarind xyloglucan | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | Gal2Xyl3Glc3glucitol prepared from tamarind flour/seed xyloglucan | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Galbeta(1-2)-Xylalpha(1-6))Glcbeta(1-4)(Galbeta(1-2)Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XLLG-ol | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzymes have 9-19times greater activities than on XXG-ol, reduced activity with XLLG-ol | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | isoenzymes have 9-19times greater activities than on XXG-ol, reduced activity with XLLG-ol | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | tamarind seed xyloglucan as donor | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | tamarind seed xyloglucan as donor | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XXXG-ol | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc-ol | XXXG-ol | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | existence of different classes of XET with differing roles in vivo | Vigna radiata var. radiata | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | existence of different classes of XET with differing roles in vivo | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | major role is the integration of new xyloglucan into the cell walls of the densely cytoplasmic florets | Brassica oleracea | ? | - |
? | |
| donor xyloglucan + xyloglucan acceptor | associated with primary cell wall metabolism, rather than mobilisation of any seed storage xyloglucan, major role is the re-structuring of existing wall material in the rapidly vacuolating shoots | Vigna radiata var. radiata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, each isoenzyme, SDS-PAGE | Vigna radiata var. radiata |
| ? | x * 32000, each isoenzyme, SDS-PAGE | Brassica oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Vigna radiata var. radiata |
| 20 | - |
assay at | Brassica oleracea |
| 24 | 34 | broad optimum | Vigna radiata var. radiata |
| 24 | 34 | broad optimum | Brassica oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
isoenzymes are heat-labile, none of the isoenzymes is particularly cold-tolerant | Vigna radiata var. radiata |
| additional information | - |
isoenzymes are heat-labile, none of the isoenzymes is particularly cold-tolerant | Brassica oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | isoenzyme C30 | Brassica oleracea |
| 5 | 5.5 | isoenzymes M35a, M35b and M55b | Vigna radiata var. radiata |
| 5 | 7 | isoenzymes M45 and M55a | Vigna radiata var. radiata |
| 6.5 | - |
isoenzymes C45a and C45b | Brassica oleracea |
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