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Literature summary for 2.4.1.205 extracted from

  • Goudsmit, E.M.; Ketchum, P.A.; Grossens, M.K.; Blake, D.A.
    Biosynthesis of galactogen: identification of a beta-(1->6)-D-galactosyltransferase in Helix pomatia albumen glands (1989), Biochim. Biophys. Acta, 992, 289-297.
    View publication on PubMed
Search for more literature for 2.4.1.205

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 UDP-D-galactose
-
 Helix pomatia

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane endomembrane-associated, probably in Golgi complex Helix pomatia 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 10 mM, 30% stimulation Helix pomatia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Helix pomatia
600000
-
 greater than 600000 Da, gel filtration Helix pomatia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPgalactose + galactogen Helix pomatia biosynthesis of galactogen UDP + 1,6-beta-D-galactosylgalactogen
-
 ?

Organism

Organism UniProt Comment Textmining
Helix pomatia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Helix pomatia

Source Tissue

Source Tissue Comment Organism Textmining
albumen gland
-
 Helix pomatia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.466
-
-
 Helix pomatia

Storage Stability

Storage Stability Organism
-80°C, crude homogenate, 6 months Helix pomatia
0°C, 24 h, 50% loss of activity Helix pomatia
4°C, purified enzyme, stable for 1 week Helix pomatia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-galactose + arabinogalactan arabinogalactan from larch wood, 22% as effective as galactogen from Helix pomatia Helix pomatia UDP + 1,6-beta-D-galactosylarabinogalactan
-
 ?
UDP-D-galactose + galactan galactan from bovine lung 64% as effective as galactogen from Helix pomatia Helix pomatia UDP + 1,6-beta-D-galactosylgalactan
-
 ?
UDP-D-galactose + galactogen galactogen from Helix pomatia best acceptor, galactogen from Lymnaea stagnalis, 22% as effective as galactogen from Helix pomatia Helix pomatia UDP + 1,6-beta-D-galactosylgalactogen
-
 ?
UDPgalactose + galactogen biosynthesis of galactogen Helix pomatia UDP + 1,6-beta-D-galactosylgalactogen
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Helix pomatia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.5
-
 Helix pomatia