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Literature summary for 2.4.1.20 extracted from

  • Liu, N.; Fosses, A.; Kampik, C.; Parsiegla, G.; Denis, Y.; Vita, N.; Fierobe, H.P.; Perret, S.
    In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum implication for cellulose catabolism (2019), Biotechnol. Biofuels, 12, 208 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Ruminiclostridium cellulolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Ruminiclostridium cellulolyticum
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
cellobiose + phosphate Ruminiclostridium cellulolyticum ATCC 35319
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Ruminiclostridium cellulolyticum
-
-
-
Ruminiclostridium cellulolyticum ATCC 35319
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Mono Q column chromatography Ruminiclostridium cellulolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + phosphate
-
 Ruminiclostridium cellulolyticum alpha-D-glucose 1-phosphate + D-glucose
-
 ?
cellobiose + phosphate
-
 Ruminiclostridium cellulolyticum ATCC 35319 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 93500, calculated from amino acid sequence Ruminiclostridium cellulolyticum

Synonyms

Synonyms Comment Organism
CbpA
-
 Ruminiclostridium cellulolyticum
cellobiose phosphorylase A
-
 Ruminiclostridium cellulolyticum

Expression

Organism Comment Expression
Ruminiclostridium cellulolyticum enzyme expression is induced during cellulose growth up

General Information

General Information Comment Organism
metabolism the enzyme is essential for growth on cellulose Ruminiclostridium cellulolyticum