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Literature summary for 2.4.1.20 extracted from

  • Yernool, D.A.; McCarthy, J.K.; Eveleigh, D.E.; Bok, J.D.
    Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana (2000), J. Bacteriol., 182, 5172-5179.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, part of oligosaccharide catabolic cluster Thermotoga neapolitana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.42
-
cellobiose
-
Thermotoga neapolitana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93000
-
SDS-PAGE Thermotoga neapolitana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + phosphate Thermotoga neapolitana nonconventional pathway for glucan utilization alpha-D-glucose 1-phosphate + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga neapolitana O52504
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from E. coli Thermotoga neapolitana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.8
-
-
Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + phosphate
-
Thermotoga neapolitana alpha-D-glucose 1-phosphate + D-glucose
-
?
cellobiose + phosphate nonconventional pathway for glucan utilization Thermotoga neapolitana alpha-D-glucose 1-phosphate + D-glucose
-
?
additional information only disaccharide accepted as substrate Thermotoga neapolitana ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermotoga neapolitana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
active for 2 h in presence of substrate, without substrate little activity after 15 min Thermotoga neapolitana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Thermotoga neapolitana