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Literature summary for 2.4.1.2 extracted from

  • Yamamoto, K.; Yoshikawa, K.; Kitahata, S.; Okada, S.
    Purification and some properties of dextrin dextranase from Acetobacter capsulatus ATCC 11894 (1992), Biosci. Biotechnol. Biochem., 56, 169-173.
No PubMed abstract available
Search for more literature for 2.4.1.2

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 0.5 mM, 83% inhibition Gluconobacter oxydans
additional information not inhibited by Ca2+, Co2+, Fe2+, Mg2+, Mn2+, Ni2+, Zn2+, p-chloromercuribenzoate and EDTA Gluconobacter oxydans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slight activation Gluconobacter oxydans
additional information no activation by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Ni2+, Zn2+ and EDTA Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
 x * 300000, SDS-PAGE Gluconobacter oxydans

Organic Solvent Stability

Organic Solvent Comment Organism
Ethylene glycol 40% v/v in acetate buffer, stable to Gluconobacter oxydans
n-Butanol H2O-saturated, 50% v/v in acetate buffer, stable to Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
 ATCC 11894
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Toyopearl, gel filtration Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.8
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m maltopentaose, maltohexaose, short-chain amylose, soluble starch Gluconobacter oxydans (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
-
 ?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m substrates are maltooligosaccharides, e.g. maltotriose (G3), maltotetraose (G4) Gluconobacter oxydans (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
-
 ?
(1,4-alpha-D-glucosyl)n + (1,6-alpha-D-glucosyl)m poor substrate: maltose (G2) Gluconobacter oxydans (1,4-alpha-D-glucosyl)n-1 + (1,6-alpha-D-glucosyl)m+1
-
 ?
maltohexaose
-
 Gluconobacter oxydans dextran dextran yield 30.2% ?
maltopentaose
-
 Gluconobacter oxydans dextran dextran yield 25.0% ?
maltotetraose
-
 Gluconobacter oxydans dextran dextran yield 13.4% ?
maltotriose
-
 Gluconobacter oxydans dextran dextran yield 11.0% ?
short-chain amylose
-
 Gluconobacter oxydans dextran dextran yield 57.6% ?
soluble starch
-
 Gluconobacter oxydans dextran dextran yield 21.4% ?

Subunits

Subunits Comment Organism
? x * 300000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 45
-
 Gluconobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
17 52 approx. half-maximal activity at 17°C and 52°C Gluconobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, stable below Gluconobacter oxydans
55
-
 inactivation within 30 min Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.2 broad optimum Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
3 5.7 about half-maximal activity at pH 3.0 and 5.7 Gluconobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 5.2 30 min stable Gluconobacter oxydans