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Literature summary for 2.4.1.198 extracted from

  • Hong, Y.; Oshihi, K.; Watanabe, R.; Endo, Y.; Maeda, Y.; Kinoshita, T.
    GPI1 stabilizes an enzyme essential in the first step of glycosylphosphatidylinositol biosynthesis (1999), J. Biol. Chem., 274, 18582-18588.
    View publication on PubMed
Search for more literature for 2.4.1.198

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of FLAG-tagged PIG-C, GST-tagged PIG-A and PIG-H with and without mGPI1 in the mGPI1-deficient F9 cells Mus musculus
mGPI1, DNA sequence determination, expression in embryonal carcinoma F9 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information disruption of the genes in F9 cells via homologous recombination, causing a severe but not complete defect in the generation of glycosylphosphatidylinositol-anchored proteins, highly reduced activity in vivo, no activity in vitro, decrease in GPI1 also caused decrease of PIG-C and PIG-H Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
endoplasmic reticulum
-
 Mus musculus 5783
-
microsome
-
 Homo sapiens
-
-
microsome
-
 Mus musculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 enzyme is active in a complex of at least 4 proteins, termed GPI1, PIG-A, PIG-C and PIG-H, in which GPI1 is absolutely essential for stabilization Homo sapiens
additional information
-
 enzyme is active in a complex of at least 4 proteins, termed GPI1, PIG-A, PIG-C and PIG-H, in which GPI1 is absolutely essential for stabilization Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is active in a complex of at least 4 proteins, termed GPI1, PIG-A, PIG-C and PIG-H, in which GPI1 is absolutely essential for stabilization ?
-
 ?
additional information Mus musculus enzyme is active in a complex of at least 4 proteins, termed GPI1, PIG-A, PIG-C and PIG-H, in which GPI1 is absolutely essential for stabilization ?
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol Homo sapiens involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol Mus musculus involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus Q9QYT7 gene mGPI1 clone
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is active in a complex of at least 4 proteins, termed GPI1, PIG-A, PIG-C and PIG-H, in which GPI1 is absolutely essential for stabilization Homo sapiens ?
-
 ?
additional information enzyme is active in a complex of at least 4 proteins, termed GPI1, PIG-A, PIG-C and PIG-H, in which GPI1 is absolutely essential for stabilization Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol enzyme complex Homo sapiens UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol enzyme complex Mus musculus UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins Homo sapiens UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?
UDP-N-acetyl-D-glucosamine + phosphatidylinositol involved in the first step of glycosylphosphatidylinositol, i.e. GPI, membrane anchor formation of surface glycoproteins Mus musculus UDP + N-acetyl-D-glucosaminyl-phosphatidylinositol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens
7.4
-
 assay at Mus musculus