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Literature summary for 2.4.1.195 extracted from
- Partial purification and characterization of Arabidopsis thaliana UDPG:thiohydroximate glucosyltransferase (1994), Phytochemistry, 36, 1133-1138.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Arabidopsis thaliana |  |
| L-cysteine | - |
Arabidopsis thaliana | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | causes 50% inhibition | Arabidopsis thaliana | |
| CuCl2 | - |
Arabidopsis thaliana | |
| additional information | iodoacetic acid and 5,5'-dithio-bis-(2-nitrobenzoic acid) are less inhibitory | Arabidopsis thaliana | |
| ZnCl2 | - |
Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
UDPglucose | pH 6.0, 30°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | activity is stimulated by thiol reducing agents | Arabidopsis thaliana | |
| MgCl2 | activity is stimulated by thiol reducing agents | Arabidopsis thaliana | |
| MnCl2 | activity is stimulated by thiol reducing agents | Arabidopsis thaliana | |
| additional information | purified enzyme lacks an absolute requirement for metal ions | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57800 | - |
- |
Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + phenylacetothiohydroximate | Arabidopsis thaliana | catalyses the penultimate reaction in glucosinolate biosynthesis | UDP + desulfoglucotropeolin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| inflorescence | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.561 | - |
- |
Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + phenylacetothiohydroximate | - |
Arabidopsis thaliana | UDP + desulfoglucotropeolin | - |
? | |
| UDPglucose + phenylacetothiohydroximate | catalyses the penultimate reaction in glucosinolate biosynthesis | Arabidopsis thaliana | UDP + desulfoglucotropeolin | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Arabidopsis thaliana |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
4.5 |
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