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Literature summary for 2.4.1.195 extracted from

  • Groot Wassink, J.W.D.; Reed, D.W.; Kolenovsky, A.D.
    Immunopurification and immunocharacterization of the glucosinolate biosynthetic enzyme thiohydroximate S-glucosyltransferase (1994), Plant Physiol., 105, 425-433.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
agriculture glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica napus
agriculture glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica oleracea
nutrition glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica napus
nutrition glucosinolates have antinutritional properties and causes acute and chronic diseases, particularly monogastrics, in domestic animals, great nutritional and therefore economic concern, since the meal fraction is directed to animal feed markets as a protein source, presence of glucosinolates in the meal precludes its use as a feed for nonruminants, this results in a worldwide effort to breed low glucosinolate varieties of rapeseed, beside traditional plant breeding there are molecular genetic studies and modification of these pathways Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55500
-
-
Brassica rapa subsp. oleifera
55500
-
-
Brassica juncea
55500
-
SDS-PAGE Brassica oleracea
57000
-
-
Brassica nigra
57600
-
gel filtration Brassica oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + phenylacetothiohydroximate Brassica napus
-
UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate Brassica oleracea
-
UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate Brassica rapa subsp. oleifera
-
UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate Brassica juncea
-
UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate Brassica nigra
-
UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate Brassica carinata
-
UDP + desulfoglucotropeolin
-
?

Organism

Organism UniProt Comment Textmining
Brassica carinata
-
-
-
Brassica juncea
-
-
-
Brassica napus
-
-
-
Brassica nigra
-
-
-
Brassica oleracea
-
cauliflower, ssp. botrytis cv Snowball, self-blanching type white and open type brown
-
Brassica rapa subsp. oleifera
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
Brassica oleracea
-
leaf
-
Brassica oleracea
-
seedling
-
Brassica napus
-
seedling
-
Brassica oleracea
-
seedling
-
Brassica juncea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2
-
-
Brassica oleracea

Storage Stability

Storage Stability Organism
-20°, crude extract stored frozen without loss of activity for 1 year Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + phenylacetothiohydroximate
-
Brassica napus UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate
-
Brassica oleracea UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate
-
Brassica rapa subsp. oleifera UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate
-
Brassica juncea UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate
-
Brassica nigra UDP + desulfoglucotropeolin
-
?
UDPglucose + phenylacetothiohydroximate
-
Brassica carinata UDP + desulfoglucotropeolin
-
?

Subunits

Subunits Comment Organism
monomer
-
Brassica napus
monomer 1 * 55500, SDS-PAGE Brassica oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Brassica oleracea isoenzymes with different pI values
-
4.8
Brassica oleracea isoenzymes with different pI values
-
4.95