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Literature summary for 2.4.1.194 extracted from

  • Bechthold, A.; Berger, U.; Heide, L.
    Partial purification, properties, and kinetic studies of UDP-glucose:p-hydroxybenzoate glucosyltransferase from cell cultures of Lithospermum erythrorhizon (1991), Arch. Biochem. Biophys., 288, 39-47.
    View publication on PubMed
Search for more literature for 2.4.1.194

General Stability

General Stability Organism
dithiothreitol stabilizes at 1-10 mM Lithospermum erythrorhizon
phenylmethylsulfonyl fluoride stabilizes at 0.02-1 mM Lithospermum erythrorhizon

Inhibitors

Inhibitors Comment Organism Structure
4-(beta-D-glucosyloxy)benzoate non competitive inhibition with respect to both 4-hidroxybenzoic acid and UDP Lithospermum erythrorhizon
Ca2+ weak effect Lithospermum erythrorhizon
Co2+ strong inhibition at 1 mM and 10 mM Lithospermum erythrorhizon
Cu2+ strong inhibition at 1 mM and 10 mM Lithospermum erythrorhizon
EDTA negative influence on activity at 10 mM, but reversible Lithospermum erythrorhizon
Fe2+ strong inhibition at 1 mM and 10 mM Lithospermum erythrorhizon
Mg2+ weak effect Lithospermum erythrorhizon
Mn2+ weak effect Lithospermum erythrorhizon
Ni2+ strong inhibition at 1 mM and 10 mM Lithospermum erythrorhizon
UDP non competitive inhibition with respect to 4-hydroxybenzoic acid, competitive inhibitor with respect to UDPglucose Lithospermum erythrorhizon
Zn2+ strong inhibition at 1 mM and 10 mM Lithospermum erythrorhizon

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.264
-
 4-hydroxybenzoate
-
 Lithospermum erythrorhizon
0.268
-
 UDPglucose
-
 Lithospermum erythrorhizon
0.6
-
 4-nitrophenol
-
 Lithospermum erythrorhizon

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ions required Lithospermum erythrorhizon

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
 apparent, from gel filtration Lithospermum erythrorhizon

Organism

Organism UniProt Comment Textmining
Lithospermum erythrorhizon
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using ammonium sulfate precipitation and chromatography on DEAE-Sepharose and phenyl-Sepharose Lithospermum erythrorhizon

Source Tissue

Source Tissue Comment Organism Textmining
callus cell culture Lithospermum erythrorhizon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00192
-
-
 Lithospermum erythrorhizon

Storage Stability

Storage Stability Organism
-20°C, 0.05 M Tris-HCl, 1 mM DTT, 0.02 mM phenylmethylsulfonyl fluoride, pH 7.6, 3 months, 10% loss of activity Lithospermum erythrorhizon
4-8°C, 0.01 M Tris-HCl, pH 7.2, 15 days, 76% loss of activity Lithospermum erythrorhizon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPglucose + 4-hydroxybenzoate
-
 Lithospermum erythrorhizon UDP + 4-(beta-D-glucosyloxy)benzoate
-
 ir
UDPglucose + 4-nitrophenol
-
 Lithospermum erythrorhizon UDP + 4-nitrophenyl O-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Lithospermum erythrorhizon

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Lithospermum erythrorhizon

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.6 half-maximal activity at pH 6.6 and 8.6 Lithospermum erythrorhizon