Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.191 extracted from

  • Schulz, M.; Weissenböck, G.
    Three specific UDP-glucuronate: flavone-glucuronosyl-transferases from primary leaves of Secale cereale (1988), Phytochemistry, 27, 1261-1267.
No PubMed abstract available
Search for more literature for 2.4.1.191

General Stability

General Stability Organism
freezing without glycerol, complete loss of activity Secale cereale

Inhibitors

Inhibitors Comment Organism Structure
luteolin 0.02 mM, 50% inhibition Secale cereale
UDP strong Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 Luteolin 7-O-diglucuronide
-
 Secale cereale
0.09
-
 UDPglucuronic acid
-
 Secale cereale

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Ca2+ or Mg2+ up to 0.5 and 0.75 mM Secale cereale

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration Secale cereale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] Secale cereale involved in the sequential biosynthesis of luteolin triglucuronide UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
-
 ir

Organism

Organism UniProt Comment Textmining
Secale cereale
-
 rye
-

Purification (Commentary)

Purification (Comment) Organism
Ultrogel AcA 44, Sephadex G-25, ultrafiltration, partial purification Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
-
 Secale cereale

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 3 months, 30-50% loss of activity Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide] 7.5% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-galactoside
-
 ?
UDP-glucose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucoside
-
 ?
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
-
 ir
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] involved in the sequential biosynthesis of luteolin triglucuronide Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
-
 ir
UDP-glucuronic acid + luteolin-7-O-glucuronide
-
 Secale cereale UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide
-
 ?
UDP-xylose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-xyloside
-
 ?
UDPglucuronic acid + luteolin-7-O-glucuronide 34% of activity with luteolin 7-O-diglucuronide Secale cereale UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
6 9 50% of maximal activity at pH 6 and pH 9 Secale cereale