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Literature summary for 2.4.1.190 extracted from

  • Schulz, M.; Weissenböck, G.
    Three specific UDP-glucuronate: flavone-glucuronosyl-transferases from primary leaves of Secale cereale (1988), Phytochemistry, 27, 1261-1267.
No PubMed abstract available
Search for more literature for 2.4.1.190

General Stability

General Stability Organism
freezing without glycerol, complete inactivation Secale cereale

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.75 mM, 40% inhibition Secale cereale
Cu2+ 0.75 mM, 40% inhibition Secale cereale
luteolin 0.1 mM, 50% inhibition Secale cereale
Pb2+ 0.75 mM, 40% inhibition Secale cereale
UDP
-
 Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 luteolin 7-O-glucuronide
-
 Secale cereale
0.04
-
 UDPglucuronic acid
-
 Secale cereale

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration Secale cereale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide Secale cereale involved in the sequential biosynthesis of luteolin triglucuronide UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?

Organism

Organism UniProt Comment Textmining
Secale cereale
-
 rye
-

Purification (Commentary)

Purification (Comment) Organism
Ultrogel AcA 44, ultrafiltration, hydroxylapatite, partial purification Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.96
-
-
 Secale cereale

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 3 months, 30-50% loss of activity Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucuronic acid + apigenin 7-O-beta-D-glucuronide 88% of activity with luteolin 7-O-glucuronide Secale cereale UDP + apigenin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
UDP-glucuronic acid + chrysoeriol 7-O-beta-D-glucuronide 65% of activity with luteolin-7-O-glucuronide Secale cereale UDP + chrysoeriol 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
-
 Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ir
UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide involved in the sequential biosynthesis of luteolin triglucuronide Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
UDPglucuronic acid + apigenin 7-O-glucoside 51.5% of activity with luteolin 7-O-glucuronide Secale cereale UDP + apigenin-7-O-beta-D-glucosyl-1,4-beta-D-glucuronoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Secale cereale
8.5
-
 pH-optimum at pH 8.5 is absent in crude enzyme preparations Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
6 9 50% of maximal activity at pH 6.0 and pH 9.0 Secale cereale

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.12
-
 UDP
-
 Secale cereale