Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.19 extracted from

  • Sonnendecker, C.; Wei, R.; Kurze, E.; Wang, J.; Oeser, T.; Zimmermann, W.
    Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli (2017), Microb. Cell Fact., 16, 87 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus sp. G-825-6

Organism

Organism UniProt Comment Textmining
Bacillus sp. G-825-6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose 6 column chromatography Bacillus sp. G-825-6

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + maltose
-
 Bacillus sp. G-825-6 cyclodextrins
-
 ?

Synonyms

Synonyms Comment Organism
CGTase
-
 Bacillus sp. G-825-6
cgtS
-
 Bacillus sp. G-825-6
cyclodextrin glucanotransferase
-
 Bacillus sp. G-825-6

Expression

Organism Comment Expression
Bacillus sp. G-825-6 the signal peptide DacD enables a 7.3fold increase of activity in the extracellular fraction after induction for 24 h compared to the native enzyme up