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Literature summary for 2.4.1.19 extracted from

  • Huang, M.; Ren, J.; Li, C.; Gu, Z.; Hong, Y.; Cheng, L.; Li, Z.
    Double mutations enhance beta-cyclization activity of cyclodextrin glycosyltransferase from Bacillus circulans (2016), J. Mol. Catal. B, 133, S100-S105 .
No PubMed abstract available
Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Niallia circulans

Protein Variants

Protein Variants Comment Organism
D577R the mutant exhibits beta-cyclization activity that is greater than that of the wild type by 3.3% Niallia circulans
Y89D the mutant exhibits total and beta-cyclization activities that are greater than that of the wild type by 14.3% and 17.6%, respectively Niallia circulans
Y89D/D577R the mutant exhibits total and beta-cyclization activities that are greater than that of the wild type by 14% and 35.1%, respectively Niallia circulans
Y89G the mutant exhibits total and beta-cyclization activities that are greater than that of the wild type by 10.6% and 14.6%, respectively Niallia circulans
Y89G/D577R the mutant exhibits total and beta-cyclization activities that are greater than that of the wild type by 25% and 29.3%, respectively Niallia circulans
Y89N the mutant exhibits total and beta-cyclization activities that are greater than that of the wild type by 8.9% and 13%, respectively Niallia circulans
Y89N/D577R the mutant exhibits total and beta-cyclization activities that are greater than that of the wild type by 19.4% and 25.2%, respectively Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans P43379
-
-
Niallia circulans STB01 P43379
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin + alpha-cylodextrin + gamma-cyclodextrin
-
 ?
maltodextrin + glycosyl acceptor
-
 Niallia circulans STB01 beta-cyclodextrin + alpha-cylodextrin + gamma-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor 45.2% conversion yield Niallia circulans beta-cyclodextrin + alpha-cylodextrin + gamma-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor 45.2% conversion yield Niallia circulans STB01 beta-cyclodextrin + alpha-cylodextrin + gamma-cyclodextrin
-
 ?

Synonyms

Synonyms Comment Organism
CGTase
-
 Niallia circulans
cyclodextrin glycosyltransferase
-
 Niallia circulans