Cloned (Comment) | Organism |
---|---|
expressed in Komagataella phaffii | Alkalihalobacillus pseudalcaliphilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus pseudalcaliphilus | - |
- |
- |
Alkalihalobacillus pseudalcaliphilus 8SB | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Alkalihalobacillus pseudalcaliphilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + glycosyl acceptor | - |
Alkalihalobacillus pseudalcaliphilus | beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
starch + glycosyl acceptor | - |
Alkalihalobacillus pseudalcaliphilus 8SB | beta-cyclodextrin + gamma-cyclodextrin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, SDS-PAGE | Alkalihalobacillus pseudalcaliphilus |
Synonyms | Comment | Organism |
---|---|---|
beta-CGTase | - |
Alkalihalobacillus pseudalcaliphilus |
cyclodextrin glucosyltransferase | - |
Alkalihalobacillus pseudalcaliphilus |
gamma-CGTase | - |
Alkalihalobacillus pseudalcaliphilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Alkalihalobacillus pseudalcaliphilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | the beta-cyclodextrin forming activity decreases to 94.6% and 89.2% after 1 and 2 h at 50°C, respectively, and decreases to 17.6% after 1 h at 70°C. After 2 h at 60°C and 60°C-70°C, the beta-cyclodextrin forming activity sharply decreases to 6% and 2.3%, respectively. After 1 and 2 h at 40°C or 50°C, the gamma-cyclodextrin forming activity slightly decreases. With the increase in temperature to 70°C, the gamma-cyclodextrin forming activity decreases to 15.2% after 1 h of treatment. Moreover, the gamma-cyclodextrin forming activity decreases to 8.9% and 2.1% after 2 h of treatment at 60°C and 70°C, respectively | Alkalihalobacillus pseudalcaliphilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | pH optimum for gamma-cyclodextrin production | Alkalihalobacillus pseudalcaliphilus |
9 | - |
pH optimum for beta-cyclodextrin production | Alkalihalobacillus pseudalcaliphilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the beta-cyclodextrin forming activity decreases with decreasing pH from 6.0 to 4.0, and increases with increasing pH from 10.0 to 11.0. However, there is no significant difference in the beta-cyclodextrin forming activity after 1 and 2 h of treatment at pH 6.0-10.0. With regard to gamma-cyclodextrin production, the enzyme activity increases with increasing pH from 4.0 to 8.0, and then decreases to 77.2% and 66.4% after 1 and 2 h at pH 11.0, respectively | Alkalihalobacillus pseudalcaliphilus |