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Literature summary for 2.4.1.19 extracted from

  • Tao, X.; Su, L.; Wang, L.; Chen, X.; Wu, J.
    Improved production of cyclodextrin glycosyltransferase from Bacillus stearothermophilus NO2 in Escherichia coli via directed evolution (2020), Appl. Microbiol. Biotechnol., 104, 173-185 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes I631T, I641T and K647E are expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
I631T the specific activities of the mutant is slightly higher than that of the wild type enzyme. The mutation has negligible effects on either the temperature and pH optima of the enzyme or its temperature and pH stability Geobacillus stearothermophilus
I641T the specific activities of the mutant is slightly higher than that of the wild type enzyme. The mutation has negligible effects on either the temperature and pH optima of the enzyme or its temperature and pH stability Geobacillus stearothermophilus
K647E the specific activities of the mutant is slightly higher than that of the wild type enzyme. The mutation has negligible effects on either the temperature and pH optima of the enzyme or its temperature and pH stability Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P31797
-
-
Geobacillus stearothermophilus NO2 P31797
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
450
-
wild type enzyme, at pH 6.0 and 50°C Geobacillus stearothermophilus
456
-
mutant enzyme I641T, at pH 6.0 and 50°C Geobacillus stearothermophilus
463
-
mutant enzyme I631T, at pH 6.0 and 50°C Geobacillus stearothermophilus
487
-
mutant enzyme K647E, at pH 6.0 and 50°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-maltoheptaoside-4-6-O-ethylidene + maltose
-
Geobacillus stearothermophilus ?
-
?
4-nitrophenyl alpha-D-maltoheptaoside-4-6-O-ethylidene + maltose
-
Geobacillus stearothermophilus NO2 ?
-
?
L-ascorbic acid + maltodextrin
-
Geobacillus stearothermophilus L-ascorbic acid-2-O-alpha-D-glucoside + ?
-
?
L-ascorbic acid + maltodextrin
-
Geobacillus stearothermophilus NO2 L-ascorbic acid-2-O-alpha-D-glucoside + ?
-
?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
CGT
-
Geobacillus stearothermophilus
CGTase
-
Geobacillus stearothermophilus
cyclodextrin glucosyltransferase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the enzyme remains stable (more than 90% activity) for 120 min at 30°C Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 more than 70% activity between pH 5.0 and 7.0 Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the enzyme remains stable for 120 min at pH 5.0-8.0 Geobacillus stearothermophilus