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Literature summary for 2.4.1.19 extracted from

  • Li, Y.; Liu, J.; Wang, Y.; Liu, B.; Xie, X.; Jia, R.; Li, C.; Li, Z.
    A two-stage temperature control strategy enhances extracellular secretion of recombinant alpha-cyclodextrin glucosyltransferase in Escherichia coli (2017), AMB Express, 7, 165 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenibacillus macerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-1,4-glucan + glycosyl acceptor Paenibacillus macerans
-
 cyclodextrins
-
 ?
alpha-1,4-glucan + glycosyl acceptor Paenibacillus macerans JFB05-01
-
 cyclodextrins
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
-
-
-
Paenibacillus macerans JFB05-01
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1,4-glucan + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrins
-
 ?
alpha-1,4-glucan + glycosyl acceptor
-
 Paenibacillus macerans JFB05-01 cyclodextrins
-
 ?
soluble starch + glycosyl acceptor
-
 Paenibacillus macerans alpha-cyclodextrin
-
 ?
soluble starch + glycosyl acceptor
-
 Paenibacillus macerans JFB05-01 alpha-cyclodextrin
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Paenibacillus macerans

Synonyms

Synonyms Comment Organism
alpha-CGTase
-
 Paenibacillus macerans
alpha-cyclodextrin glucosyltransferase
-
 Paenibacillus macerans

Expression

Organism Comment Expression
Paenibacillus macerans when the enzyme expression is induced at 25°C for 32 h, and then the temperature is shifted to 30°C, the extracellular enzyme activity at 90 h is 45% higher than that observed when induction is performed at a constant temperature of 25°C up