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Literature summary for 2.4.1.19 extracted from
- Structural basis of a mutant Y195I alpha-cyclodextrin glycosyltransferase with switched product specificity from alpha-cyclodextrin to beta-/gamma-cyclodextrin (2014), J. Biotechnol., 182-183, 92-96.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant Y195I is expressed in Escherichia coli BL21(DE3) cells | Paenibacillus macerans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant Y195I, hanging drop vapor diffusion method, using 0.2 M sodium acetate trihydrate, 0.1 M Tris hydrochloride pH 9.0, 30% (w/v) polyethylene glycol 4000, at 22°C | Paenibacillus macerans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y195I | the mutation drastically alters the cyclodextrin specificity of the enzyme by switching toward the synthesis of both beta- and gamma-cyclodextrins | Paenibacillus macerans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + glycosyl acceptor | Paenibacillus macerans | - |
cyclodextrins | the main product is alpha-cyclodextrin | ? |  |
| starch + glycosyl acceptor | Paenibacillus macerans 602-1 | - |
cyclodextrins | the main product is alpha-cyclodextrin | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | - |
- |
- |
| Paenibacillus macerans 602-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography and Sephacryl-100 gel filtration | Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | the main product is alpha-cyclodextrin | ? | |
| starch + glycosyl acceptor | - |
Paenibacillus macerans 602-1 | cyclodextrins | the main product is alpha-cyclodextrin | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-CGTase | - |
Paenibacillus macerans |
| alpha-cyclodextrin glycosyltransferase | - |
Paenibacillus macerans |
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Release 2023.1 (January 2023)
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