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Literature summary for 2.4.1.19 extracted from
- Purification and characterization of cyclodextrin beta-glucanotransferase from novel alkalophilic bacilli (2015), Bioprocess Biosyst. Eng., 38, 767-776.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | about 30% residual activity at 1 mM | Alkalihalobacillus lehensis |  |
| Ba2+ | about 88% residual activity at 1 mM | Alkalihalobacillus lehensis | |
| Ca2+ | about 90% residual activity at 1 mM | Alkalihalobacillus lehensis | |
| Cd2+ | about 96% residual activity at 1 mM | Alkalihalobacillus lehensis | |
| K+ | about 38% residual activity at 1 mM | Alkalihalobacillus lehensis | |
| Mg2+ | about 40% residual activity at 1 mM | Alkalihalobacillus lehensis | |
| Mn2+ | about 95% residual activity at 1 mM | Alkalihalobacillus lehensis | |
| Zn2+ | about 28% residual activity at 1 mM | Alkalihalobacillus lehensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5% enhancement of activity at 1 mM Cu2+ | Alkalihalobacillus lehensis | |
| Fe2+ | 9% enhancement of activity at 1 mM Fe2+ | Alkalihalobacillus lehensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74100 | - |
x * 74100, SDS-PAGE | Alkalihalobacillus lehensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alkalihalobacillus lehensis | - |
- |
- |
| Alkalihalobacillus lehensis MLB2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration | Alkalihalobacillus lehensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.21 | - |
crude enzyme, at pH 7.0 and 60°C | Alkalihalobacillus lehensis |
| 57.7 | - |
after 274fold purification, at pH 7.0 and 60°C | Alkalihalobacillus lehensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextrin + glycosyl acceptor | - |
Alkalihalobacillus lehensis | beta-cyclodextrin | - |
? | |
| dextrin + glycosyl acceptor | - |
Alkalihalobacillus lehensis MLB2 | beta-cyclodextrin | - |
? | |
| additional information | no activity with D-glucose | Alkalihalobacillus lehensis | ? | - |
? | |
| additional information | no activity with D-glucose | Alkalihalobacillus lehensis MLB2 | ? | - |
? | |
| potato starch + glycosyl acceptor | - |
Alkalihalobacillus lehensis | beta-cyclodextrin | - |
? | |
| potato starch + glycosyl acceptor | - |
Alkalihalobacillus lehensis MLB2 | beta-cyclodextrin | - |
? | |
| rice starch + glycosyl acceptor | highest activity | Alkalihalobacillus lehensis | beta-cyclodextrin | - |
? | |
| rice starch + glycosyl acceptor | highest activity | Alkalihalobacillus lehensis MLB2 | beta-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Alkalihalobacillus lehensis | beta-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Alkalihalobacillus lehensis MLB2 | beta-cyclodextrin | - |
? | |
| sweet potato starch + glycosyl acceptor | - |
Alkalihalobacillus lehensis | beta-cyclodextrin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74100, SDS-PAGE | Alkalihalobacillus lehensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CGTase | - |
Alkalihalobacillus lehensis |
| cyclodextrin beta-glucanotransferase | - |
Alkalihalobacillus lehensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Alkalihalobacillus lehensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | more than 70% activity between 50 and 70°C | Alkalihalobacillus lehensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | after 1 h of incubation, the enzyme shows about 43% activity at 40°C, about 80% at 50°C, about 90% at 55°C, 100% at 60°C, about 78% at 70°C, about 35% at 80°C, and about 20% activity at 90°C | Alkalihalobacillus lehensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Alkalihalobacillus lehensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | more than 50% activity between pH 5.0 and 8.0 | Alkalihalobacillus lehensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | after 1 h of incubation, the enzyme shows less than 20% activity at pH 4.0 and 5.0, about 35% at pH 6.0, 100% at pH 7.0, about 80% at pH 8.0, about 30% at pH 9.0, and about 20% activity at pH 10.0 | Alkalihalobacillus lehensis |
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