Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Bacillus subtilis strain DB104A | Niallia circulans |
Protein Variants | Comment | Organism |
---|---|---|
A230V | the mutation confers increased resistance to inhibition by acarbose, the mutant shows highly increased IC50 compared to the wild-type enzyme | Niallia circulans |
F283L | the mutation confers increased resistance to inhibition by acarbose, the mutant shows highly increased IC50 compared to the wild-type enzyme | Niallia circulans |
H140Q | the mutation confers increased resistance to inhibition by acarbose, the mutant shows highly increased IC50 compared to the wild-type enzyme | Niallia circulans |
K232E | the mutation confers increased resistance to inhibition by acarbose, the mutant shows highly increased IC50 compared to the wild-type enzyme | Niallia circulans |
additional information | error-prone PCR mutagenesis for searching the sequence space of CGTase for acarbose-insensitive variants, overview | Niallia circulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | the acceptor substrate binding site near the active site of the enzyme is involved in inhibition by acarbose, mutants K232E, F283L, and A230V show increased resistance, overview | Niallia circulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Niallia circulans | P30920 | - |
- |
Niallia circulans 251 | P30920 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | beta-cyclodextrin-forming activity from partially hydrolyzed potato starch with an average degree of polymerization of 50. Disproportionation activity is determined using 4-nitrophenyl-beta-D-maltoheptaoside-4-6-O-ethylidene, i.e. pNPG7, as substrate | Niallia circulans | ? | - |
? | |
additional information | beta-cyclodextrin-forming activity from partially hydrolyzed potato starch with an average degree of polymerization of 50. Disproportionation activity is determined using 4-nitrophenyl-beta-D-maltoheptaoside-4-6-O-ethylidene, i.e. pNPG7, as substrate | Niallia circulans 251 | ? | - |
? | |
starch | - |
Niallia circulans | beta-cyclodextrin + gamma-cyclodextrin | - |
? | |
starch | - |
Niallia circulans 251 | beta-cyclodextrin + gamma-cyclodextrin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Niallia circulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Niallia circulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
starch | pH 6.0, 60°C, beta-cyclization activity, mutant A230V | Niallia circulans | |
26 | - |
starch | pH 6.0, 60°C, beta-cyclization activity, mutant K232E | Niallia circulans | |
35 | - |
starch | pH 6.0, 60°C, beta-cyclization activity, mutant F283L | Niallia circulans | |
48 | - |
starch | pH 6.0, 60°C, beta-cyclization activity, mutant A230V/H140Q | Niallia circulans | |
79 | - |
starch | pH 6.0, 60°C, beta-cyclization activity, mutant H140Q | Niallia circulans | |
329 | - |
starch | pH 6.0, 60°C, beta-cyclization activity, wild-type enzyme | Niallia circulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Niallia circulans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0011 | - |
pH 6.0, 60°C, wild-type enzyme | Niallia circulans | acarbose | |
1.807 | - |
pH 6.0, 60°C, mutant H140Q | Niallia circulans | acarbose | |
1.845 | - |
pH 6.0, 60°C, mutant A230V/H140Q | Niallia circulans | acarbose | |
3.93 | - |
pH 6.0, 60°C, mutant K232E | Niallia circulans | acarbose | |
4.197 | - |
pH 6.0, 60°C, mutant F283L | Niallia circulans | acarbose | |
7.37 | - |
pH 6.0, 60°C, mutant A230V | Niallia circulans | acarbose |