KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.54 | - |
beta-cyclodextrin | pH 8.5, 50°C | Bacillus sp. (in: Bacteria) | |
15.54 | - |
soluble starch | pH 8.5, 50°C | Bacillus sp. (in: Bacteria) | |
15.54 | - |
4-nitrophenyl-beta-D-glucopyranose | pH 8.5, 50°C | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) BL-12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 11.4fold from strain BL-12 by ultrafiltration and beta-cyclodextrin polymer affinity chromatography | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
205 | - |
purified enzyme | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyclodextrin + 4-nitrophenyl-beta-D-glucopyranose | beta-cyclodextrin as a glycosyl donor and 4-nitrophenyl-beta-D-glucopyranose as a glycosyl acceptor | Bacillus sp. (in: Bacteria) | ? | - |
? | |
beta-cyclodextrin + 4-nitrophenyl-beta-D-glucopyranose | beta-cyclodextrin as a glycosyl donor and 4-nitrophenyl-beta-D-glucopyranose as a glycosyl acceptor | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
additional information | glycosyl donor specificity for intermolecular transglycosylation of stevioside, overview, cyclization reaction with soluble starch as substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | glycosyl donor specificity for intermolecular transglycosylation of stevioside, overview, cyclization reaction with soluble starch as substrate | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
stevioside + maltodextrin | the enzyme from strain BL-12 is more suitable for transglycosylation than the cyclization reaction, and is specific for the intermolecular transglycosylation of stevioside with maltodextrin as the most suitable glycosyl donor | Bacillus sp. (in: Bacteria) | ? | - |
? | |
stevioside + maltodextrin | the enzyme from strain BL-12 is more suitable for transglycosylation than the cyclization reaction, and is specific for the intermolecular transglycosylation of stevioside with maltodextrin as the most suitable glycosyl donor | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? | |
stevioside + starch | soluble, extrusion, or potato starch | Bacillus sp. (in: Bacteria) | ? | - |
? | |
stevioside + starch | soluble, extrusion, or potato starch | Bacillus sp. (in: Bacteria) BL-12 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
beta-CGTase | - |
Bacillus sp. (in: Bacteria) |
beta-cyclodextrin glucanotransferase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | - |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified native enzyme, thermal stability at 40°C is elevated 10fold in the presence of 1% maltodextrin | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Bacillus sp. (in: Bacteria) |