Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Priestia megaterium | |
EDTA | - |
Priestia megaterium | |
Hg+ | - |
Priestia megaterium | |
Zn2+ | - |
Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Priestia megaterium | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Priestia megaterium | |
Cu2+ | activates | Priestia megaterium | |
Mg2+ | activates | Priestia megaterium | |
Mn2+ | activates | Priestia megaterium | |
additional information | enzyme activity is not affected by Ca2+, Na+, K+, Fe3+, ICH2COOH, and SDS at low concentration | Priestia megaterium | |
Sr2+ | activates | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68200 | - |
gel filtration | Priestia megaterium |
73400 | - |
1 * 73400, SDS-PAGE | Priestia megaterium |
75000 | - |
about, mass spectrometry | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by adsorption on starch, ion exchange chromatography, and gel filtration | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60.39 | - |
purified enzyme | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme preforms cyclization of different alpha-1,4-glucans, e.g. soluble potato starch, or amylopectin, and amylose, the enzyme forms preferably beta-cyclodextrins, the ratio of products is 27:68:5 for alpha, beta, and gamma cyclodextrins | Priestia megaterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 73400, SDS-PAGE | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
CGTase | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | activity range | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, about 60% of maximal activity at pH 4.5-9.0 | Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | stable | Priestia megaterium |