Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.19 extracted from

  • Rashid, N.; Cornista, J.; Ezaki, S.; Fukui, T.; Atomi, H.; Imanaka, T.
    Characterization of an archaeal cyclodextrin glucanotransferase with a novel C-terminal domain (2002), J. Bacteriol., 184, 777-784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Niallia circulans
-
Thermoanaerobacterium thermosulfurigenes
-
Paenibacillus macerans
-
Geobacillus stearothermophilus
-
Thermococcus sp.
-
Klebsiella oxytoca
pET-8c vector, gene cloned and expressed in Escherichia coli BL21(DE3) Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Niallia circulans
-
Thermoanaerobacterium thermosulfurigenes

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Bacillus sp. (in: Bacteria)
Ca2+
-
Niallia circulans
Ca2+ thermal stability is remarkably increased by addition of calcium ions Geobacillus stearothermophilus
Ca2+ thermal stability is remarkably increased by addition of calcium ions Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
SDS-PAGE Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
1011
-
Bacillus sp. (in: Bacteria) 1011
-
1011
-
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus P31797
-
-
Klebsiella oxytoca P08704
-
-
Niallia circulans
-
-
-
Niallia circulans
-
B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
Niallia circulans P43379
-
-
Niallia circulans 251
-
-
-
Niallia circulans 251 P43379
-
-
Paenibacillus macerans P31835
-
-
Thermoanaerobacterium thermosulfurigenes
-
EM1
-
Thermoanaerobacterium thermosulfurigenes EM1
-
EM1
-
Thermococcus kodakarensis Q8X268 KOD1, UniProt accession No. Q8X268
-
Thermococcus sp.
-
B1001
-
Thermococcus sp. Q9UWN2 B1001
-
Thermococcus sp. B1001
-
B1001
-
Thermococcus sp. B1001 Q9UWN2 B1001
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + H2O
-
Thermococcus kodakarensis cyclodextrins
-
?
additional information
-
Geobacillus stearothermophilus ?
-
?
additional information
-
Niallia circulans ?
-
?
additional information pullulan is no substrate Thermococcus kodakarensis ?
-
?
additional information
-
Niallia circulans 251 ?
-
?
soluble starch + glycosyl acceptor
-
Geobacillus stearothermophilus alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Thermococcus sp. alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Thermococcus kodakarensis alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) 1011 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Thermococcus sp. B1001 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans 251 alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Thermococcus kodakarensis beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Bacillus sp. (in: Bacteria) 1011 beta-cyclodextrin
-
r
soluble starch + glycosyl acceptor
-
Niallia circulans 251 beta-cyclodextrin
-
r
soluble starch + H2O
-
Niallia circulans cyclodextrins
-
?
soluble starch + H2O
-
Thermococcus kodakarensis cyclodextrins
-
?
soluble starch + H2O
-
Niallia circulans 251 cyclodextrins
-
?

Synonyms

Synonyms Comment Organism
CGT_TK
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Geobacillus stearothermophilus
75
-
recombinant enzyme in absence of Ca2+ Thermococcus kodakarensis
80
-
-
Geobacillus stearothermophilus
80
-
-
Thermococcus kodakarensis
85 90 in presence of Ca2+ Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85 100 extreme thermostability with addition of Ca2+, no loss of activity after 80 min at 85°C, half-life of 20 min at 100°C, recombinant enzyme half-life of 40 min at 100°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Thermococcus kodakarensis