Cloned (Comment) | Organism |
---|---|
- |
Niallia circulans |
- |
Thermoanaerobacterium thermosulfurigenes |
- |
Paenibacillus macerans |
- |
Geobacillus stearothermophilus |
- |
Thermococcus sp. |
- |
Klebsiella oxytoca |
pET-8c vector, gene cloned and expressed in Escherichia coli BL21(DE3) | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
- |
Niallia circulans |
- |
Thermoanaerobacterium thermosulfurigenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus sp. (in: Bacteria) | |
Ca2+ | - |
Niallia circulans | |
Ca2+ | thermal stability is remarkably increased by addition of calcium ions | Geobacillus stearothermophilus | |
Ca2+ | thermal stability is remarkably increased by addition of calcium ions | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
SDS-PAGE | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
1011 | - |
Bacillus sp. (in: Bacteria) 1011 | - |
1011 | - |
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus | P31797 | - |
- |
Klebsiella oxytoca | P08704 | - |
- |
Niallia circulans | - |
- |
- |
Niallia circulans | - |
B. sp. var. alkalophilus No. 38-2, ATCC 21783 | - |
Niallia circulans | P43379 | - |
- |
Niallia circulans 251 | - |
- |
- |
Niallia circulans 251 | P43379 | - |
- |
Paenibacillus macerans | P31835 | - |
- |
Thermoanaerobacterium thermosulfurigenes | - |
EM1 | - |
Thermoanaerobacterium thermosulfurigenes EM1 | - |
EM1 | - |
Thermococcus kodakarensis | Q8X268 | KOD1, UniProt accession No. Q8X268 | - |
Thermococcus sp. | - |
B1001 | - |
Thermococcus sp. | Q9UWN2 | B1001 | - |
Thermococcus sp. B1001 | - |
B1001 | - |
Thermococcus sp. B1001 | Q9UWN2 | B1001 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | - |
Thermococcus kodakarensis | cyclodextrins | - |
? | |
additional information | - |
Geobacillus stearothermophilus | ? | - |
? | |
additional information | - |
Niallia circulans | ? | - |
? | |
additional information | pullulan is no substrate | Thermococcus kodakarensis | ? | - |
? | |
additional information | - |
Niallia circulans 251 | ? | - |
? | |
soluble starch + glycosyl acceptor | - |
Geobacillus stearothermophilus | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Thermococcus sp. | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Thermococcus kodakarensis | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) 1011 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Thermococcus sp. B1001 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans 251 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Thermococcus kodakarensis | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) 1011 | beta-cyclodextrin | - |
r | |
soluble starch + glycosyl acceptor | - |
Niallia circulans 251 | beta-cyclodextrin | - |
r | |
soluble starch + H2O | - |
Niallia circulans | cyclodextrins | - |
? | |
soluble starch + H2O | - |
Thermococcus kodakarensis | cyclodextrins | - |
? | |
soluble starch + H2O | - |
Niallia circulans 251 | cyclodextrins | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGT_TK | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Geobacillus stearothermophilus |
75 | - |
recombinant enzyme in absence of Ca2+ | Thermococcus kodakarensis |
80 | - |
- |
Geobacillus stearothermophilus |
80 | - |
- |
Thermococcus kodakarensis |
85 | 90 | in presence of Ca2+ | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 100 | extreme thermostability with addition of Ca2+, no loss of activity after 80 min at 85°C, half-life of 20 min at 100°C, recombinant enzyme half-life of 40 min at 100°C | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
Thermococcus kodakarensis |