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Literature summary for 2.4.1.19 extracted from
- Purification and properties of cyclomaltodextrin glucanotransferase from an alkalophilic Bacillus (1988), Biotechnol. Appl. Biochem., 10, 414-427.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | ATCC 21783, enzyme is becoming commercially important since cyclodextrins have found various practical applications | Niallia circulans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
- |
Niallia circulans |
- |
Paenibacillus macerans |
General Stability
| General Stability | Organism |
|---|---|
| ATCC 2178, exceptionally stable | Niallia circulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
strain ATCC 21783, gel filtration | Niallia circulans |
| 70500 | - |
ATCC 21783, SDS-PAGE | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
1011 | - |
| Bacillus sp. (in: Bacteria) 1011 | - |
1011 | - |
| Klebsiella pneumoniae | - |
- |
- |
| Niallia circulans | - |
B. sp. var. alkalophilus No. 38-2, ATCC 21783 | - |
| Paenibacillus macerans | - |
IAM1243 | - |
| Paenibacillus macerans IAM1243 | - |
IAM1243 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenibacillus macerans |
| ATCC 21783 | Niallia circulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1650 | - |
ATCC 21783 | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltoheptaose + glycosyl acceptor | ATCC 21783 | Niallia circulans | beta-cyclodextrin | - |
? |  |
| maltopentaose + glycosyl acceptor | ATCC 21783 | Niallia circulans | beta-cyclodextrin | - |
r | |
| maltotetraose + glycosyl acceptor | ATCC 21783 | Niallia circulans | beta-cyclodextrin | - |
r | |
| maltotriose + glycosyl acceptor | ATCC 21783 | Niallia circulans | beta-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | ATCC 21783 | Niallia circulans | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) 1011 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans IAM1243 | alpha-cyclodextrin + beta cyclodextrin + gamma-cyclodextrin | - |
? | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) | alpha-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | alpha-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Bacillus sp. (in: Bacteria) 1011 | alpha-cyclodextrin | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans IAM1243 | alpha-cyclodextrin | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 | Niallia circulans |
| 7 | - |
- |
Paenibacillus macerans |
| 7 | - |
ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 | Niallia circulans |
| 9.5 | - |
ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 | Niallia circulans |
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