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Literature summary for 2.4.1.19 extracted from
- Immobilization of starch-degrading enzymes. I. A comparative study on soluble and immobilized cyclodextrin glycosyltransferase (1983), J. Appl. Biochem., 5, 158-164.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | application of immobilized enzyme is practically important for continous production of cyclodextrins, broad activity maximum is advantageous for industrial operations, immobilized enzyme is able to work at maximum efficiency at lower temperatures | Paenibacillus macerans |
General Stability
| General Stability | Organism |
|---|---|
| immobilization in strongly hydrophilic microenvironment markedly enhances conformational stability in a wide temperature and pH range | Paenibacillus macerans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus macerans | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity after dissolution and dialysis 28 units/mg protein, 1 unit is the amount of enzyme which gives a linear increase of 1% transmission per minute at 40°C, immobilized enzyme 230-450 units/g solid | Paenibacillus macerans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen + acceptor | - |
Paenibacillus macerans | beta-cyclodextrin | - |
r |  |
| maltooligosaccharides + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r | |
| soluble starch + glycosyl acceptor | - |
Paenibacillus macerans | cyclodextrins | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Akrilex C cyclodextrin glycosyltransferase | immobilized enzyme | Paenibacillus macerans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | immobilized enzyme | Paenibacillus macerans |
| 60 | - |
- |
Paenibacillus macerans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | immobilized enzyme is stable at 40°C, heat inactivation above 50°C | Paenibacillus macerans |
| 50 | 70 | immobilized enzyme is more stable than soluble enzyme, inactivation of the soluble enzyme at 50°C is 1.6 times, at 60°C is 6.9 times and at 70°C is 24.3 times faster | Paenibacillus macerans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
immobilized enzyme | Paenibacillus macerans |
| 5.9 | - |
- |
Paenibacillus macerans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | - |
Paenibacillus macerans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Paenibacillus macerans |
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