Cloned (Comment) | Organism |
---|---|
cell-free expression of His-tagged wild-type and mutant enzymes | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | non-glucosylated glycogenin-1 constructs, with various amino acid substitutions in position 83 and 195, are expressed in a cell-free expression system and autoglucosylated in vitro | Homo sapiens |
T83A | site-directed mutagenesis, the mutant shows no incorporation of glucose, no autoglycosylation | Homo sapiens |
T83C | site-directed mutagenesis, the mutant shows no incorporation of glucose, no autoglycosylation | Homo sapiens |
T83F | site-directed mutagenesis, the mutant shows no incorporation of glucose, no autoglycosylation | Homo sapiens |
T83M | naturally occuring mutation, glycogenin-1 with the Thr83Met substitution is unable to form the glucose-O-tyrosine linkage at tyrosine 195 unless co-expressed with the catalytically active Tyr195Phe glycogenin-1. The mutant shows no incorporation of glucose, no autoglycosylation | Homo sapiens |
T83S | site-directed mutagenesis, the mutant is catalytically active | Homo sapiens |
T83V | site-directed mutagenesis, the mutant shows no incorporation of glucose, no autoglycosylation | Homo sapiens |
T83Y | site-directed mutagenesis, the mutant shows no incorporation of glucose, no autoglycosylation | Homo sapiens |
Y195F | site-directed mutagenesis, glycogenin-1 with the Thr83Met substitution is unable to form the glucose-O-tyrosine linkage at tyrosine 195 unless co-expressed with the catalytically active Tyr195Phe glycogenin-1 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
recombinant His-tagged unglucosylated glycogenin-1, gel filtration | Homo sapiens |
41000 | - |
recombinant His-tagged autoglucosylated glycogenin-1, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin | Homo sapiens | autoglucosylation by glycogenin-1 | UDP + alpha-D-glucosylglycogenin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | in vitro autoglucosylation of wild-type and mutant enzymes | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from cell-free expression by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | glycogenin-1 catalyzes both the glucose-O-tyrosine linkage and the alpha1,4 glucosidic bonds linking the glucose molecules in the oligosaccharide | Homo sapiens | ? | - |
? | |
UDP-alpha-D-glucose + glycogenin | autoglucosylation by glycogenin-1 | Homo sapiens | UDP + alpha-D-glucosylglycogenin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogenin-1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | glycogen depletion in skeletal muscle is a result of a non-functional glycogenin-1 due to a Thr83Met substitution in glycogenin-1 | Homo sapiens |
additional information | aggregation might be an explanation for the incomplete autoglucosylation of wild-type glycogenin-1 | Homo sapiens |
physiological function | glycogenin-1 initiates the glycogen synthesis in skeletal muscle by the autocatalytic formation of a short oligosaccharide at tyrosine 195 | Homo sapiens |