Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a GST-tagged protein | Neurospora crassa |
Protein Variants | Comment | Organism |
---|---|---|
DELTA306-664 | 7fold increase in self-glucosylation | Neurospora crassa |
DELTA306-664/Y196F | no self-glucosylation activity | Neurospora crassa |
DELTA306-664/Y196F/Y198F | expression results in no accumulation of glycogen | Neurospora crassa |
DELTA360-664 | 11.8fold increase in self-glucosylation | Neurospora crassa |
DELTA360-664/Y196F | expression results in reduced glycogen accumulation to 30% of the wild-type enzyme, very low self-glucosylation activity | Neurospora crassa |
DELTA360-664/Y196F/Y198F | no self-glucosylation activity | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00441 | - |
UDP-glucose | - |
Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogenin | Neurospora crassa | self-glucosylating initiator of glycogen synthesis | UDP + glucosylglycogenin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q6Q2C8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | two glucosylation sites Tyr196 and Tyr198. The enzyme is a self-glucosylating initiator of glycogen synthesis, recombinant enzyme contains an oligosaccharide chain attached to Tyr196 | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogenin | - |
Neurospora crassa | UDP + glucosylglycogenin | - |
? | |
UDP-glucose + glycogenin | self-glucosylating initiator of glycogen synthesis | Neurospora crassa | UDP + glucosylglycogenin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GNN | - |
Neurospora crassa |