Literature summary for 2.4.1.186 extracted from

de Paula, R.M.; Wilson, W.A.; Roach, P.J.; Terenzi, H.F.; Bertolini, M.C.
Biochemical characterization of Neurospora crassa glycogenin (GNN), the self-glucosylating initiator of glycogen synthesis (2005), FEBS Lett., 579, 2208-2214.

Search for more literature for 2.4.1.186

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as a GST-tagged protein	Neurospora crassa

Protein Variants

Protein Variants	Comment	Organism
DELTA306-664	7fold increase in self-glucosylation	Neurospora crassa
DELTA306-664/Y196F	no self-glucosylation activity	Neurospora crassa
DELTA306-664/Y196F/Y198F	expression results in no accumulation of glycogen	Neurospora crassa
DELTA360-664	11.8fold increase in self-glucosylation	Neurospora crassa
DELTA360-664/Y196F	expression results in reduced glycogen accumulation to 30% of the wild-type enzyme, very low self-glucosylation activity	Neurospora crassa
DELTA360-664/Y196F/Y198F	no self-glucosylation activity	Neurospora crassa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00441	-	UDP-glucose	-	Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + glycogenin	Neurospora crassa	self-glucosylating initiator of glycogen synthesis	UDP + glucosylglycogenin	-	?

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	Q6Q2C8	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	two glucosylation sites Tyr196 and Tyr198. The enzyme is a self-glucosylating initiator of glycogen synthesis, recombinant enzyme contains an oligosaccharide chain attached to Tyr196	Neurospora crassa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + glycogenin	-	Neurospora crassa	UDP + glucosylglycogenin	-	?
UDP-glucose + glycogenin	self-glucosylating initiator of glycogen synthesis	Neurospora crassa	UDP + glucosylglycogenin	-	?

Synonyms

Synonyms	Comment	Organism
GNN	-	Neurospora crassa

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Release 2023.1 (January 2023)