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Literature summary for 2.4.1.186 extracted from

  • Goldraij, A.; Curtino, J.A.
    M-glycogenin, the protein moiety of Neurospora crassa proteoglycogen, is an auto- and transglucosylating enzyme (1996), Biochem. Biophys. Res. Commun., 227, 909-914.
    View publication on PubMed

General Stability

General Stability Organism
loss of activity on Q-Sepharose during ion exchange chromatography Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Neurospora crassa
phosphate slight inhibition at 100 mM Neurospora crassa
UDP inhibits both autoglucosylation and glucosylation of exogenous acceptor Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ can not substitute Mn2+ Neurospora crassa
Mn2+ dependent on, also required for transglucosylation Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
SDS-PAGE Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + glycogenin Escherichia coli enzyme forms the protein part of proteoglycogen UDP + glucosylated glycogenin
-
?
UDP-glucose + glycogenin Neurospora crassa enzyme forms the protein part of proteoglycogen UDP + glucosylated glycogenin
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Neurospora crassa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein autoglycosylation Escherichia coli
glycoprotein autoglycosylation Neurospora crassa

Purification (Commentary)

Purification (Comment) Organism
separation from proteoglycogen Neurospora crassa

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin independent active sites for glucosylation of exogenous and self-acceptors Neurospora crassa
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Escherichia coli
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Neurospora crassa
-

Storage Stability

Storage Stability Organism
-20°C, amylolyzed M-glycogenin preparation, at least 1 month without appreciable loss of activity Neurospora crassa
4°C, amylolyzed M-glycogenin preparation, at least 1 week without appreciable loss of activity Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + glycogenin autoglycosylation reaction Escherichia coli UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
UDP-glucose + glycogenin autoglycosylation reaction Neurospora crassa UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Escherichia coli UDP + glucosylated glycogenin
-
?
UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Neurospora crassa UDP + glucosylated glycogenin
-
?
UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Escherichia coli UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Neurospora crassa UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
UDP-glucose + glycogenin UDP-glucose can not be replaced by ADP- or GDP-glucose Neurospora crassa UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
UDP-glucose + n-dodecyl-beta-D-maltoside transglucosylation reaction Escherichia coli UDP + n-dodecyl-beta-D-maltotriose
-
?
UDP-glucose + n-dodecyl-beta-D-maltoside transglucosylation reaction Neurospora crassa UDP + n-dodecyl-beta-D-maltotriose
-
?
UDP-glucose + n-dodecyl-beta-D-maltoside simultaneously and independently of the autoglycosylation reaction Escherichia coli UDP + n-dodecyl-beta-D-maltotriose
-
?
UDP-glucose + n-dodecyl-beta-D-maltoside simultaneously and independently of the autoglycosylation reaction Neurospora crassa UDP + n-dodecyl-beta-D-maltotriose
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Neurospora crassa
More enzyme forms the protein part of proteoglycogen Escherichia coli
More enzyme forms the protein part of proteoglycogen Neurospora crassa

Synonyms

Synonyms Comment Organism
M-glycogenin
-
Escherichia coli
M-glycogenin
-
Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Neurospora crassa