BRENDA - Enzyme Database show
show all sequences of 2.4.1.184

Free fatty acids regulate two galactosyltransferases in chloroplast envelope membranes isolated from spinach leaves

Sakaki, T.; Kondo, N.; Yamada, M.; Plant Physiol. 94, 781-787 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
alpha-linolenic acid
0.3 mM, 28fold stimulation in the presence of 2 mM MgCl2, MgCl2 can be replaced by MnCl2, CaCl2 or high concetrations of KCl
Spinacia oleracea
hexadecatrienoic acid
approx. 3fold activation
Spinacia oleracea
linoleic acid
approx. 4fold activiation
Spinacia oleracea
oleic acid
approx. 4fold activiation
Spinacia oleracea
palmitic acid
approx. 2fold activiation
Spinacia oleracea
palmitoleic acid
approx. 4fold activiation
Spinacia oleracea
stearic acid
approx. 2fold activiation
Spinacia oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast envelope
envelope
Spinacia oleracea
9941
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
alpha-linolenic acid stimulates activity in presence of CaCl2
Spinacia oleracea
K+
alpha-linolenic acid stimulates in presence of high concentrations of KCl
Spinacia oleracea
Mg2+
alpha-linolenic acid causes drastic increase in activity under limiting concentrations of MgCl2, without affecting its maximum activity at higher MgCl2 concentration, free alpha-linolenic acid alone does not affect the activity; EDTA inhibits stimulation; unsaturated 16- and 18-carbon fatty acids stimulate in presence of MgCl2
Spinacia oleracea
Mn2+
alpha-linolenic acid stimulates activity in presence of MnCl2
Spinacia oleracea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
cv. New Asia
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
488815
Spinacia oleracea
1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + 1,2-diacyl-sn-glycerol
-
488815
Spinacia oleracea
-
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
alpha-linolenic acid
0.3 mM, 28fold stimulation in the presence of 2 mM MgCl2, MgCl2 can be replaced by MnCl2, CaCl2 or high concetrations of KCl
Spinacia oleracea
hexadecatrienoic acid
approx. 3fold activation
Spinacia oleracea
linoleic acid
approx. 4fold activiation
Spinacia oleracea
oleic acid
approx. 4fold activiation
Spinacia oleracea
palmitic acid
approx. 2fold activiation
Spinacia oleracea
palmitoleic acid
approx. 4fold activiation
Spinacia oleracea
stearic acid
approx. 2fold activiation
Spinacia oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast envelope
envelope
Spinacia oleracea
9941
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
alpha-linolenic acid stimulates activity in presence of CaCl2
Spinacia oleracea
K+
alpha-linolenic acid stimulates in presence of high concentrations of KCl
Spinacia oleracea
Mg2+
alpha-linolenic acid causes drastic increase in activity under limiting concentrations of MgCl2, without affecting its maximum activity at higher MgCl2 concentration, free alpha-linolenic acid alone does not affect the activity; EDTA inhibits stimulation; unsaturated 16- and 18-carbon fatty acids stimulate in presence of MgCl2
Spinacia oleracea
Mn2+
alpha-linolenic acid stimulates activity in presence of MnCl2
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
488815
Spinacia oleracea
1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + 1,2-diacyl-sn-glycerol
-
488815
Spinacia oleracea
-
Other publictions for EC 2.4.1.184
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
659479
Benning
Three enzyme systems for galac ...
Arabidopsis thaliana
J. Biol. Chem.
280
2397-2400
2005
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1
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676458
Hoelzl
Glycoengineering of cyanobacte ...
Chloroflexus aurantiacus
Plant Cell Physiol.
46
1766-1778
2005
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1
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660126
Kelly
Disruption of the two digalact ...
Arabidopsis thaliana
Plant Cell
15
2694-2706
2003
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1
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1
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1
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3
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1
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1
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1
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1
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1
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488815
Sakaki
Free fatty acids regulate two ...
Spinacia oleracea
Plant Physiol.
94
781-787
1990
7
-
-
-
-
-
-
-
2
4
-
-
-
2
-
-
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1
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1
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7
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-
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2
4
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-
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-
-
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1
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-
1
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-
-
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-
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-
-
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488814
Heemskerk
-
UDPgalactose-independent synth ...
Spinacia oleracea
Biochim. Biophys. Acta
961
38-47
1988
-
-
-
-
-
-
2
-
1
1
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1
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1
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2
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2
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1
1
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1
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2
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-
-
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488813
Heemskerk
-
Characterization of galactosyl ...
Spinacia oleracea
Biochim. Biophys. Acta
918
189-203
1987
1
-
-
-
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-
6
-
1
7
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1
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-
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1
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1
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1
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6
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1
7
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1
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1
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486286
Heemskerk
-
Localization of galactolipid:g ...
Spinacia oleracea
Biochim. Biophys. Acta
877
281-289
1986
-
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-
-
-
-
-
-
2
-
-
-
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1
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1
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2
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1
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-
-
-
-
-
-
-
-
-
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488812
Heemskerk
-
Turnover of galactolipids inco ...
Spinacia oleracea
Biochim. Biophys. Acta
754
181-189
1983
-
-
-
-
-
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1
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1
5
-
-
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1
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1
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1
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1
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1
1
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1
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1
5
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1
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1
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1
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1
1
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-
-
-
-
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488811
Dorne
-
The galactolipid:galactolipid ...
Spinacia oleracea
FEBS Lett.
145
30-34
1982
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1
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1
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1
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2
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2
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1
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