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Literature summary for 2.4.1.183 extracted from
- Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall alpha-1,3-glucan synthase (2016), Biosci. Biotechnol. Biochem., 80, 1853-1863 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively | Aspergillus oryzae |
| additional information | disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. Mutant phenotypes, overview. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively | Aspergillus oryzae |
| additional information | disruption of the genes encoding cell wall alpha-1,3-glucan synthases, i.e. agsA, agsB, agsC, in Aspergillus oryzae strain NS4 (sC-, niaD-) leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. Transformation of the cutinase-encoding gene cutL1 into Aspergillus oryzae wild-type and the tripleDELTA mutant showing that tripleDELTA-cutL1 forms smaller hyphal pellets and shows both greater biomass and increased CutL1 productivity compared to wild-type-cutL1, which might be attributable to a decrease in the number of tripleDELTA-cutL1 cells under anaerobic conditions. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain. Analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + [alpha-D-glucosyl-(1->3)]n | Aspergillus oryzae | - |
UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? |  |
| UDP-glucose + [alpha-D-glucosyl-(1->3)]n | Aspergillus oryzae RIB 40 | - |
UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
| UDP-glucose + [alpha-D-glucosyl-(1->3)]n | Aspergillus oryzae ATCC 42149 | - |
UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | Q2TXK6 | - |
- |
| Aspergillus oryzae | Q2UEQ6 | - |
- |
| Aspergillus oryzae | Q2UIS4 | - |
- |
| Aspergillus oryzae ATCC 42149 | Q2TXK6 | - |
- |
| Aspergillus oryzae ATCC 42149 | Q2UEQ6 | - |
- |
| Aspergillus oryzae ATCC 42149 | Q2UIS4 | - |
- |
| Aspergillus oryzae RIB 40 | Q2TXK6 | - |
- |
| Aspergillus oryzae RIB 40 | Q2UEQ6 | - |
- |
| Aspergillus oryzae RIB 40 | Q2UIS4 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus oryzae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + [alpha-D-glucosyl-(1->3)]n | - |
Aspergillus oryzae | UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
| UDP-glucose + [alpha-D-glucosyl-(1->3)]n | - |
Aspergillus oryzae RIB 40 | UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
| UDP-glucose + [alpha-D-glucosyl-(1->3)]n | - |
Aspergillus oryzae ATCC 42149 | UDP + [alpha-D-glucosyl-(1->3)]n+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agsA | - |
Aspergillus oryzae |
| agsB | - |
Aspergillus oryzae |
| agsC | - |
Aspergillus oryzae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains iapproximately twice that produced by the wild-type-cutL1 strain | Aspergillus oryzae |
| malfunction | disruption of the genes encoding cell wall alpha-1,3-glucan synthase leads to increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae. The amount of CutL1 secreted by the tripleDELTA-cutL1 and quintupleDELTA- cutL1 strains is approximately twice that produced by the wild-type-cutL1 strain | Aspergillus oryzae |
| additional information | analysis of the monosaccharide composition of the cell walls from wild-type, tripleDELTA (agsADELTAagsBDELTAagsCDELTA), and quintupleDELTA (agsADELTAagsBDELTAagsCDELTAamyGDELTA), lyophilized hyphal cells of each strain of Aspergillus oryzae, overview. The HW fraction mainly contains galactose and mannose and the AS1 fraction mainly contains glucose and mannose. The alkali-soluble 2 (AS2) fraction derived from Aspergillus oryzae wild-type mainly contains glucose (approx. 20% of the AS2 fraction) with a small amount of mannose. But those derived from Aspergillus oryzae tripleDELTA and quintupleDELTA contain significantly less glucose. The alkali-soluble 1 (A1) fraction derived from each strain contains glucose, glucosamine, and mannose. The glucose and glucosamine contents of the A1 fraction derived from each strain are around 28% and 20% of the total dry weight of the A1 fraction, respectively | Aspergillus oryzae |
| physiological function | the ags genes play a major role in alpha-1,3-glucan biosynthesis in Aspergillus oryzae, while the amyD and amyG genes do not | Aspergillus oryzae |
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