Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.183 extracted from

  • Jolly, L.; Newell, J.; Porcelli, I.; Vincent, S.J.F.; Stingele, F.
    Lactobacillus helveticus glycosyltransferases: from genes to carbohydrate synthesis (2002), Glycobiology, 12, 319-327.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of eps gene cluster from genomic library and expression in Escherichia coli as His-tagges or GST-tagged proteins, DNA sequence analysis Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus
-
-
-
Lactobacillus helveticus
-
gene epsF
-
Lactobacillus helveticus NCC2745
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant as His-tagged or GST-tagged proteins from Escherichia coli Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
assay development Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester Lactobacillus helveticus ?
-
?
additional information no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester Lactobacillus helveticus NCC2745 ?
-
?
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37 Lactobacillus helveticus UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose
-
?
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37 Lactobacillus helveticus NCC2745 UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose
-
?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n substrate specificity Lactobacillus helveticus UDP + [alpha-D-glucosyl-(1-3)]n+1 product analysis ?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate Lactobacillus helveticus UDP + [alpha-D-glucosyl-(1-3)]n+1 product analysis ?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain Lactobacillus helveticus UDP + [alpha-D-glucosyl-(1-3)]n+1 product analysis ?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n substrate specificity Lactobacillus helveticus NCC2745 UDP + [alpha-D-glucosyl-(1-3)]n+1 product analysis ?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate Lactobacillus helveticus NCC2745 UDP + [alpha-D-glucosyl-(1-3)]n+1 product analysis ?
UDP-glucose + [alpha-D-glucosyl-(1-3)]n enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain Lactobacillus helveticus NCC2745 UDP + [alpha-D-glucosyl-(1-3)]n+1 product analysis ?