Cloned (Comment) | Organism |
---|---|
cloning of eps gene cluster from genomic library and expression in Escherichia coli as His-tagges or GST-tagged proteins, DNA sequence analysis | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | - |
- |
- |
Lactobacillus helveticus | - |
gene epsF | - |
Lactobacillus helveticus NCC2745 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant as His-tagged or GST-tagged proteins from Escherichia coli | Lactobacillus helveticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay development | Lactobacillus helveticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester | Lactobacillus helveticus | ? | - |
? | |
additional information | no activity with p-aminophenyl, p-nitrophenyl, and 6-(fluorescein-5-carboxamido)-hexanoic acid succimidyl ester | Lactobacillus helveticus NCC2745 | ? | - |
? | |
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose | synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37 | Lactobacillus helveticus | UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose | - |
? | |
UDP-glucose + 2-(octadecylthio)-ethyl-beta-D-glucose | synthetic acceptor composed of a monosaccharide linked to a long hydrophobic aliphatic chain, -CH2CH2SC18H37 | Lactobacillus helveticus NCC2745 | UDP + 2-(octadecylthio)-ethyl-beta-D-glucose-1,3-alpha-D-glucose | - |
? | |
UDP-glucose + [alpha-D-glucosyl-(1-3)]n | substrate specificity | Lactobacillus helveticus | UDP + [alpha-D-glucosyl-(1-3)]n+1 | product analysis | ? | |
UDP-glucose + [alpha-D-glucosyl-(1-3)]n | enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate | Lactobacillus helveticus | UDP + [alpha-D-glucosyl-(1-3)]n+1 | product analysis | ? | |
UDP-glucose + [alpha-D-glucosyl-(1-3)]n | enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain | Lactobacillus helveticus | UDP + [alpha-D-glucosyl-(1-3)]n+1 | product analysis | ? | |
UDP-glucose + [alpha-D-glucosyl-(1-3)]n | substrate specificity | Lactobacillus helveticus NCC2745 | UDP + [alpha-D-glucosyl-(1-3)]n+1 | product analysis | ? | |
UDP-glucose + [alpha-D-glucosyl-(1-3)]n | enzyme forms alpha-1,3- and alpha-1,6-linkages onto the initial beta-D-glucose-phosphate | Lactobacillus helveticus NCC2745 | UDP + [alpha-D-glucosyl-(1-3)]n+1 | product analysis | ? | |
UDP-glucose + [alpha-D-glucosyl-(1-3)]n | enzyme EpsF: introduction of an alpha-1,3-linkage branch at the first beta-D-glucose-phosphate of the side-chain | Lactobacillus helveticus NCC2745 | UDP + [alpha-D-glucosyl-(1-3)]n+1 | product analysis | ? |