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Literature summary for 2.4.1.182 extracted from
- Purification and characterization of the lipid A disaccharide synthase (LpxB) from Escherichia coli, a peripheral membrane protein (2009), Biochemistry, 48, 11559-11571.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | between 0 and 0.5 mM Triton X-100 increases the apparent specific activity 5fold | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain XL1-Blue | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D89A | mutant shows no residual catalytic activity | Escherichia coli |
| R201A | mutant shows no residual catalytic activity | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | LpxB activity is dependent upon the bulk surface concentration of ist substrates in a mixed micelle assay system, suggesting that catalysis occurs at the membrane interface | Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Haemophilus influenzae |
| 43000 | - |
8 * 43000, SDS-PAGE | Escherichia coli |
| 360000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli C41(DE3) | - |
- |
- |
| Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45 | - |
enzyme from membrane-free lysate, at 30°C | Escherichia coli |
| 183 | - |
after 4.0fold purification, at 30°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate | - |
Haemophilus influenzae | 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP | - |
? | |
| UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate | - |
Escherichia coli | 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP | - |
? | |
| UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate | - |
Escherichia coli C41(DE3) | 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homooctamer | 8 * 43000, SDS-PAGE | Escherichia coli |
| monomer | 1 * 42000, SDS-PAGE | Haemophilus influenzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lipid A disaccharide synthase | - |
Haemophilus influenzae |
| lipid A disaccharide synthase | - |
Escherichia coli |
| LpxB | - |
Haemophilus influenzae |
| LpxB | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | catalyzes the fifth step of lipid A biosynthesis | Haemophilus influenzae |
| metabolism | catalyzes the fifth step of lipid A biosynthesis | Escherichia coli |
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