Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Vibrio vulnificus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His Trap column chromatography | Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin | - |
Vibrio vulnificus | amylopectin containing alpha-1,6-glucosidic linkages | - |
? | |
corn starch | - |
Vibrio vulnificus | corn starch containing alpha-1,6-glucosidic linkages | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GBE | - |
Vibrio vulnificus |
glycogen branching enzyme | - |
Vibrio vulnificus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Vibrio vulnificus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | more than 70% activity between 20 and 40°C, less than 40% activity at 45°C, less than 10% activity at 50°C | Vibrio vulnificus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the half-life of the enzyme at 35°C is 10 h | Vibrio vulnificus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vibrio vulnificus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | about 60% activity at pH 6.5, about 80% activity at pH 7.0, 100% activity at pH 7.5, more than 90% between pH 8.0, and 11.0 | Vibrio vulnificus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | more than 50% of activity is retained when the enzyme is incubated at 4°C and pH 5.5-10.5 for 48 h,with the enzyme being most stable at pH 9.5 | Vibrio vulnificus |