Literature summary for 2.4.1.18 extracted from

Ban, X.; Dhoble, A.S.; Li, C.; Zhang, Y.; Gu, Z.; Cheng, L.; Hong, Y.; Li, Z.
Potassium and sodium ions enhance the activity and thermostability of 1,4-alpha-glucan branching enzyme from Geobacillus thermoglucosidasius in the presence of glycerol (2017), Int. J. Biol. Macromol., 102, 712-717 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
glycerol	103.9% activity at 10% (v/v)	Parageobacillus thermoglucosidasius

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Parageobacillus thermoglucosidasius

General Stability

General Stability	Organism
the addition of glycerol further increases the thermostability of the enzyme	Parageobacillus thermoglucosidasius

Inhibitors

Inhibitors	Comment	Organism	Structure
glycerol	high concentration of glycerol decreases the branching activity of the enzyme (97.9% residual activity at 30% (v/v)	Parageobacillus thermoglucosidasius

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	K+ increases branching activity of the enzyme when concentration is less than 0.5 mM (127.9% activity at 0.5 mM). In the presence of glycerol, K+ significantly enhances the thermostability and maintains the structures of the enzyme. K+ with 10% (v/v) glycerol increases branching activity of the enzyme by 31.7%	Parageobacillus thermoglucosidasius
Na+	Na+ increases branching activity of the enzyme when concentration is less than 1.0 mM (114.4% activity at 1.0 mM). In the presence of glycerol, Na+ significantly enhances the thermostability and maintains the structures of the enzyme	Parageobacillus thermoglucosidasius

Organism

Organism	UniProt	Comment	Textmining
Parageobacillus thermoglucosidasius	A0A068F9H3	-	-
Parageobacillus thermoglucosidasius STB02	A0A068F9H3	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap phenyl column chromatography and HiTrap ANX column chromatography	Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amylose	-	Parageobacillus thermoglucosidasius	amylose containing alpha-1,6-glucosidic linkages	-	?
amylose	-	Parageobacillus thermoglucosidasius STB02	amylose containing alpha-1,6-glucosidic linkages	-	?

Synonyms

Synonyms	Comment	Organism
GBE	-	Parageobacillus thermoglucosidasius

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Release 2023.1 (January 2023)