Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Oryza sativa |
Crystallization (Comment) | Organism |
---|---|
sitting drop method, 20°C | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
D270A | mutant with about 10% relative enzyme activity compared to wild-type enzyme | Oryza sativa |
D344A | mutant without enzyme activity | Oryza sativa |
E399A | mutant with about 5% relative enzyme activity compared to wild-type enzyme | Oryza sativa |
G468D | mutant with about 95% relative enzyme activity compared to wild-type enzyme | Oryza sativa |
H275A | mutant without enzyme activity | Oryza sativa |
H467A | mutant without enzyme activity | Oryza sativa |
R342A | mutant with about 15% relative enzyme activity compared to wild-type enzyme | Oryza sativa |
Y235A | mutant with about 15% relative enzyme activity compared to wild-type enzyme | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q01401 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-sepharose chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
2.5 U/mg for amylopectin, 1 U = decrease of one absorbance unit at 660 nm at 30°C | Oryza sativa |
additional information | - |
20.8 U/mg for amylose, 1 U = decrease of one absorbance unit at 660 nm at 30°C | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin | assay at 30°C, reaction terminated by heating at 95°C for 5 min | Oryza sativa | ? | - |
? | |
amylose | assay at 30°C, reaction terminated by heating at 95°C for 5 min | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BEI | - |
Oryza sativa |
starch branching enzyme I | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Oryza sativa |