Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Bovine liver non-specific transfer proteins | activation | Homo sapiens | |
CHAPSO | 5 to 7fold less effective compared to Triton CF-54 | Homo sapiens | |
deoxycholate | 5 to 7fold less effective compared to Triton CF-54 | Homo sapiens | |
additional information | little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A | Homo sapiens | |
phosphatidylethanolamine | activation, less efficient than Triton CF-54 | Homo sapiens | |
taurodeoxycholate | 5 to 7fold less effective compared to Triton CF-54 | Homo sapiens | |
Triton CF-54 | activation, optimal at 0.1% | Homo sapiens | |
Triton X-100 | activation, can replace Triton CF-54 with 75% less efficiency | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
lactotriaosylceramide | - |
Homo sapiens | |
0.048 | - |
UDP-galactose | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Golgi apparatus | - |
Homo sapiens | 5794 | - |
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, can replace Mn2+ with 26% efficiency | Homo sapiens | |
Co2+ | activation, can replace Mn2+ with 51% efficiency | Homo sapiens | |
Cu2+ | activation, can replace Mn2+ with 15% efficiency | Homo sapiens | |
Mg2+ | activation, can replace Mn2+ with 13% efficiency | Homo sapiens | |
Mn2+ | requirement, 25 mM | Homo sapiens | |
additional information | no activation by Zn2+ or Cd2+ | Homo sapiens | |
Ni2+ | activation, can replace Mn2+ with 16% efficiency | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid | Homo sapiens | associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure | UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from Colo 205 cell line | Homo sapiens |
partial | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Homo sapiens | - |
Colo-205 cell | colonic adenocarcino cell line | Homo sapiens | - |
colonic adenocarcinoma cell | - |
Homo sapiens | - |
SW-403 cell | colonic adenocarcino cell line | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00077 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid | associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure | Homo sapiens | UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid | - |
? | |
UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R | substrate specificity | Homo sapiens | UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R | - |
? | |
UDP-galactose + lactotriaosylceramide | best substrate | Homo sapiens | UDP + lactotetraosylceramide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Hepes buffer | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.2 | about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer | Homo sapiens |