BRENDA - Enzyme Database
show all sequences of 2.4.1.178

Biosynthesis of cyanogenic glucosides: in vitro analysis of the glucosylation step

Hösel, W.; Schiel, O.; Arch. Biochem. Biophys. 229, 177-186 (1984)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
UDP-glucose
4-hydroxymandelonitrile or 3,4-dihydroxymandelonitrile
Triglochin maritima
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Triglochin maritima
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
High salt concentrations
160% activity observed at 0.6 M concentration of NaCl, KCl or ammonium sulfate with 4-hydroxymandelonitrile, and 20% activity observed at 0.1-0.2 M concentration with 3,4-dihydroxymandelonitrile
Triglochin maritima
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
1 * 47000, SDS-PAGE
Triglochin maritima
50000
-
gel filtration
Triglochin maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP-glucose + 4-hydroxymandelonitrile
Triglochin maritima
last step in biosynthesis of cyanogenic glucosides
UDP + taxiphyllin
-
Triglochin maritima
?
Organism
Organism
UniProt
Commentary
Textmining
Triglochin maritima
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
method that includes chromatography on Sephadex PD10 and DEAE-Sephadex
Triglochin maritima
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Triglochin maritima
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.31
-
3,4-dihydroxymandelonitrile
Triglochin maritima
0.94
-
4-hydroxymandelonitrile
Triglochin maritima
Storage Stability
Storage Stability
Organism
-20°C, 10% glycerol, crude extract stable for several weeks
Triglochin maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-glucose + 3,4-dihydroxymandelonitrile
-
587060
Triglochin maritima
UDP + 3,4-dihydroxymandelonitrile glucoside
-
587060
Triglochin maritima
?
UDP-glucose + 4-hydroxymandelonitrile
-
587060
Triglochin maritima
UDP + taxiphyllin
-
587060
Triglochin maritima
?
UDP-glucose + 4-hydroxymandelonitrile
last step in biosynthesis of cyanogenic glucosides
587060
Triglochin maritima
UDP + taxiphyllin
-
587060
Triglochin maritima
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 47000, SDS-PAGE
Triglochin maritima
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Triglochin maritima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
8.5
-
Triglochin maritima
8
-
assay at
Triglochin maritima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
about 50% of activity maximum at pH 6 and pH 9.5
Triglochin maritima
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
-
unstable below, crude enzyme extract
Triglochin maritima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.5
-
UDP-glucose
4-hydroxymandelonitrile or 3,4-dihydroxymandelonitrile
Triglochin maritima
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Triglochin maritima
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
High salt concentrations
160% activity observed at 0.6 M concentration of NaCl, KCl or ammonium sulfate with 4-hydroxymandelonitrile, and 20% activity observed at 0.1-0.2 M concentration with 3,4-dihydroxymandelonitrile
Triglochin maritima
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
47000
-
1 * 47000, SDS-PAGE
Triglochin maritima
50000
-
gel filtration
Triglochin maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP-glucose + 4-hydroxymandelonitrile
Triglochin maritima
last step in biosynthesis of cyanogenic glucosides
UDP + taxiphyllin
-
Triglochin maritima
?
Purification (Commentary) (protein specific)
Commentary
Organism
method that includes chromatography on Sephadex PD10 and DEAE-Sephadex
Triglochin maritima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Triglochin maritima
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.31
-
3,4-dihydroxymandelonitrile
Triglochin maritima
0.94
-
4-hydroxymandelonitrile
Triglochin maritima
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10% glycerol, crude extract stable for several weeks
Triglochin maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-glucose + 3,4-dihydroxymandelonitrile
-
587060
Triglochin maritima
UDP + 3,4-dihydroxymandelonitrile glucoside
-
587060
Triglochin maritima
?
UDP-glucose + 4-hydroxymandelonitrile
-
587060
Triglochin maritima
UDP + taxiphyllin
-
587060
Triglochin maritima
?
UDP-glucose + 4-hydroxymandelonitrile
last step in biosynthesis of cyanogenic glucosides
587060
Triglochin maritima
UDP + taxiphyllin
-
587060
Triglochin maritima
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 47000, SDS-PAGE
Triglochin maritima
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Triglochin maritima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
8.5
-
Triglochin maritima
8
-
assay at
Triglochin maritima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9.5
about 50% of activity maximum at pH 6 and pH 9.5
Triglochin maritima
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
-
unstable below, crude enzyme extract
Triglochin maritima
Other publictions for EC 2.4.1.178
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
587060
Hösel
Biosynthesis of cyanogenic glu ...
Triglochin maritima
Arch. Biochem. Biophys.
229
177-186
1984
-
-
-
-
-
-
-
1
1
1
2
1
-
1
-
-
1
-
-
1
2
1
3
1
-
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
2
1
-
-
-
1
-
1
2
1
3
1
1
-
-
-
2
1
1
-
-
-
-
-
-
-
639554
Poulton
-
Prunasin biosynthesis by cell- ...
Prunus serotina
Z. Naturforsch. C
38
369-374
1983
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
5
1
5
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
5
1
5
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-