Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.177 extracted from

  • Gottardi, M.; Gruen, P.; Bode, H.B.; Hoffmann, T.; Schwab, W.; Oreb, M.; Boles, E.
    Optimisation of trans-cinnamic acid and hydrocinnamyl alcohol production with recombinant Saccharomyces cerevisiae and identification of cinnamyl methyl ketone as a by-product (2017), FEMS Yeast Res., 17, fox091 .
    View publication on PubMed
Search for more literature for 2.4.1.177

Application

Application Comment Organism
synthesis trans-cinnamic acid (tCA) and hydrocinnamyl alcohol (HcinOH) are valuable aromatic compounds with applications in the flavour, fragrance and cosmetic industry. Production in transgenic Saccharomyces cerevisiae strain strain MGY0.06 expressing phenylalanine ammonia lyase (PAL, from Photorhabdus luminescens) and an aryl carboxylic acid reductase, as well as UDP-glucose:cinnamate glucosyltransferase FaGT2 from from Fragaria ananassa. Overview of the heterologous pathway Fragaria x ananassa

Cloned(Commentary)

Cloned (Comment) Organism
gene GT2, recombinant expression in Saccharomyces cerevisiae strain CEN.PK113-7D, coexpression with phenylalanine ammonia lyase (PAL, from Photorhabdus luminescens) and an aryl carboxylic acid reductase, resulting in strain MGY0.06 Fragaria x ananassa

Protein Variants

Protein Variants Comment Organism
additional information production of trans-cinnamic acid (tCA) and hydrocinnamyl alcohol (HcinOH) in transgenic Saccharomyces cerevisiae strain MGY0.06 expressing phenylalanine ammonia lyase (PAL, from Photorhabdus luminescens) and an aryl carboxylic acid reductase, as well as UDP-glucose:cinnamate glucosyltransferase FaGT2 from from Fragaria ananassa. Overview of the heterologous pathway. Optimisation of the biosynthesis pathway for tCA and HcinOH production Fragaria x ananassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + trans-cinnamate Fragaria x ananassa
-
 UDP + trans-cinnamoyl beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa Q66PF4 i.e. Fragaria chiloensis x Fragaria virginiana
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit green immature strawberry fruits are the main source of gallic acid, beta-glucogallin, and ellagic acid in accordance with the highest GT2 gene expression levels Fragaria x ananassa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows substrate promiscuity as it forms glucose esters of a variety of (hydroxyl)benzoic and (hydroxyl)cinnamic acids Fragaria x ananassa ?
-
-
UDP-glucose + trans-cinnamate
-
 Fragaria x ananassa UDP + trans-cinnamoyl beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
FaGT2
-
 Fragaria x ananassa
UDP-glucose:cinnamate glucosyltransferase
-
 Fragaria x ananassa

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the formation of 1-O-galloyl-beta-D-glucopyranose (beta-glucogallin) the precursor of ellagitannin biosynthesis Fragaria x ananassa