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Literature summary for 2.4.1.177 extracted from

  • Shimizu, T.; Kojima, M.
    Partial purification and characterization of UDPG:t-cinnamate glucosyltransferase in the root of sweet potato, Ipomoea batatas Lam (1984), J. Biochem., 95, 205-212.
    View publication on PubMed

General Stability

General Stability Organism
PMSF and 2-mercaptoethanol stabilize during purification Ipomoea batatas
freezing inactivates, sorbitol stabilizes Ipomoea batatas
sorbitol, 10%, stabilizes during storage Ipomoea batatas

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 strong Ipomoea batatas
additional information no inhibition by MgCl2, CaCl2, MnCl2, chlorogenic acid (1 mM each) Ipomoea batatas
p-chloromercuribenzoate
-
Ipomoea batatas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
UDP-glucose
-
Ipomoea batatas
0.2
-
trans-cinnamate
-
Ipomoea batatas

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Ipomoea batatas
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by MgCl2, CaCl2 or MnCl2, 1 mM each Ipomoea batatas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
gel filtration Ipomoea batatas

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + cinnamate Ipomoea batatas involved in biosynthesis of chlorogenic acid in root of Ipomoea batatas UDP + 1-O-cinnamoyl-beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Ipomoea batatas
-
sweet potato, Lam cv. Norin 1
-

Purification (Commentary)

Purification (Comment) Organism
partial Ipomoea batatas

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Ipomoea batatas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70.1
-
-
Ipomoea batatas

Storage Stability

Storage Stability Organism
-20°C, 10% sorbitol, about 10% loss of activity within 30 days Ipomoea batatas
sorbitol, 10%, stabilizes during storage Ipomoea batatas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + cinnamate best substrate Ipomoea batatas UDP + 1-O-cinnamoyl-beta-D-glucose trans-isomer ?
UDP-alpha-D-glucose + cinnamate trans-cinnamate Ipomoea batatas UDP + 1-O-cinnamoyl-beta-D-glucose trans-isomer ?
UDP-alpha-D-glucose + cinnamate involved in biosynthesis of chlorogenic acid in root of Ipomoea batatas Ipomoea batatas UDP + 1-O-cinnamoyl-beta-D-glucose
-
?
UDP-glucose + p-coumarate the glucosyl-group is introduced exclusively into the carboxyl-group Ipomoea batatas UDP + p-coumaroyl-D-glucose
-
?
UDP-glucose + p-coumarate glucosylation at 57% the rate of cinnamate Ipomoea batatas UDP + p-coumaroyl-D-glucose
-
?
UDP-glucose + 4-hydroxy-3-methoxycinnamate glucosylation at 35% the rate of cinnamate Ipomoea batatas UDP + 4-hydroxy-3-methoxycinnamoyl beta-D-glucoside
-
?
UDP-glucose + o-coumarate glucosylation at 52% the rate of cinnamate Ipomoea batatas UDP + o-coumaroyl beta-D-glucose
-
?
UDP-glucose + benzoate glucosylation at 71% the rate of cinnamate Ipomoea batatas UDP + benzoyl-beta-D-glucoside
-
?
UDP-alpha-D-glucose + ferulate glucosylation at 27% the rate of cinnamate Ipomoea batatas UDP + 1-O-feruloyl-beta-D-glucose
-
?
UDP-alpha-D-glucose + caffeate glucosylation at 15% the rate of cinnamate Ipomoea batatas UDP + 1-O-caffeoyl-beta-D-glucose
-
?
UDP-glucose + trans-cinnamate
-
Ipomoea batatas UDP + trans-cinnamoyl beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ipomoea batatas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Ipomoea batatas