Any feedback?
Literature summary for 2.4.1.175 extracted from
- Chondroitin sulfate synthase-2/chondroitin polymerizing factor has two variants with distinct function (2010), J. Biol. Chem., 285, 34155-34167.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| individual overexpression of soluble forms of FLAG- and Myc-tagged splice variants CSS2A and CSS2B in COS-7 cells, co-expression with CSS1, EC 2.4.1.174. Whereas CSS2A facilitates chondroitin sulfate biosynthesis, CSS2B inhibits it. Analysis of transcription levels of CSS2 variants | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | both splice variants CSS2A and CSS2B | Mus musculus | 5783 | - |
| Golgi apparatus | both splice variants CSS2A and CSS2B | Mus musculus | 5794 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Mus musculus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
two CSS2 splice variants, CSS2A and CSS2B | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| fibroblast | embryonic, expression level analysis for the splice variants CSS2A and CSS2B, overview | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000006 | - |
recombinant CSS2B variant in COS-7 cells, pH 6.2, 37°C | Mus musculus |
| 0.000008 | - |
recombinant CSS2A variant in COS-7 cells, pH 6.2, 37°C | Mus musculus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homology modeling of the N-terminal domain of CSS2 variants, three-dimensional structure of the N-terminal domain of CSS2 variants showing differences in the catalytic sites, overview | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chondroitin sulfate synthase-2/chondroitin polymerizing factor | - |
Mus musculus |
| CSS2 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | reduced glucuronyltransferase activity for splice variant CSS2B and no polymerizing activity for CSS2B co-expressed with CSS1, in contrast to splice variant CSS2A co-expressed with CSS1 | Mus musculus |
| additional information | either of CSS2A, CSS2B, and CSS1/ChSy-1 heterogeneously and homogeneously interact with each other, suggesting that they form a complex of multimers | Mus musculus |
| physiological function | CSS2 is involved in elongation of chondroitin sulfate chains. Splice variant CSS2A facilitates chondroitin sulfate biosynthesis, while splice variant CSS2B inhibits it, molecular modeling and mechanisms of chondroitin sulfate biosynthesis regulation, overview. The ratio of CSS2 variants correlates with age-dependent change of chondroitin sulfate chain length in brain of mice | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
