Literature summary for 2.4.1.175 extracted from

Osawa, T.; Sugiura, N.; Shimada, H.; Hirooka, R.; Tsuji, A.; Shirakawa, T.; Fukuyama, K.; Kimura, M.; Kimata, K.; Kakuta, Y.
Crystal structure of chondroitin polymerase from Escherichia coli K4 (2009), Biochem. Biophys. Res. Commun., 378, 10-14.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
K4CP in the presence of UDP and UDP-N-acetylgalactosamine as well as with UDP and UDP-glucuronic acid, hanging drop vapor diffusion method and batch method, using 15-20% (w/v) PEG 3350 and 200 mM NaCl	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide	Escherichia coli	-	UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide	-	?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide	Escherichia coli K4	-	UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q8L0V4	-	-
Escherichia coli K4	Q8L0V4	-	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose column chromatography and Superdex 200 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide	-	Escherichia coli	UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide	-	?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide	-	Escherichia coli K4	UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide	-	?

Synonyms

Synonyms	Comment	Organism
chondroitin polymerase	-	Escherichia coli
K4CP	-	Escherichia coli

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Release 2023.1 (January 2023)