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Literature summary for 2.4.1.175 extracted from
- Chondroitin sulfate synthase-3. Molecular cloning and characterization (2003), J. Biol. Chem., 278, 39711-39725.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in COS cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.221 | - |
UDP-GalNAc | - |
Homo sapiens |  |
| 0.36 | - |
chondroitin sulfate CS-11 | - |
Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | type II membrane protein | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | activates | Homo sapiens | |
| Co2+ | divalent cation is essential, Co2+ evokes the highest level of activity | Homo sapiens | |
| Mn2+ | 31% as effective as Co2 in reaction with chondroitin sulfate C11 and UDP-glucuronic acid, 64% as effective as Co2+ in reaction with chondroitin sulfate C10 and UDP-GalNAc | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | CSS3 is a glycosyltransferase having both glucuronyltransferase and N-acetylgalactosaminyltransferase activities. It may make a contribution to CS biosynthesis that differs from that of CSS1 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q70JA7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | very low expression level | Homo sapiens | - |
| bone marrow | very low expression level | Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| cerebral cortex | - |
Homo sapiens | - |
| colon | very low expression level | Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | very low expression level | Homo sapiens | - |
| leukocyte | very low expression level | Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| mammary gland | - |
Homo sapiens | - |
| ovary | - |
Homo sapiens | - |
| pancreas | - |
Homo sapiens | - |
| placenta | very low expression level | Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| small intestine | - |
Homo sapiens | - |
| stomach | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| thymus | very low expression level | Homo sapiens | - |
| thyroid gland | very low expression level | Homo sapiens | - |
| uterus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin + UDP-GalNAc | chondroitin is the best substrate | Homo sapiens | ? | - |
? | |
| chondroitin + UDP-glucuronic acid | chondroitin is the best substrate | Homo sapiens | ? | - |
? | |
| chondroitin sulfate A + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate A + UDP-glucuronic acid | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate A10 + UDP-GalNAc | no activity with UDP-glucuronic acid | Homo sapiens | ? | - |
? | |
| chondroitin sulfate A11 + UDP-glucuronic acid | no activity with UDP-GalNAc | Homo sapiens | ? | - |
? | |
| chondroitin sulfate C + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate C + UDP-glucuronic acid | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate C10 + UDP-GalNAc | no activity with UDP-glucuronic acid | Homo sapiens | ? | - |
? | |
| chondroitin sulfate C11 + UDP-glucuronic acid | no activity with UDP-GalNAc | Homo sapiens | ? | - |
? | |
| chondroitin sulfate D + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate D + UDP-glucuronic acid | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate E + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
| chondroitin sulfate E + UDP-glucuronic acid | - |
Homo sapiens | ? | - |
? | |
| dermatan sulfate + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
| dermatan sulfate + UDP-glucuronic acid | - |
Homo sapiens | ? | - |
? | |
| hyaluronan + UDP-GalNAc | - |
Homo sapiens | ? | - |
? | |
| hyaluronan + UDP-glucuronic acid | - |
Homo sapiens | ? | - |
? | |
| additional information | CSS3 is a glycosyltransferase having both glucuronyltransferase and N-acetylgalactosaminyltransferase activities. It may make a contribution to CS biosynthesis that differs from that of CSS1 | Homo sapiens | ? | - |
? | |
| additional information | glucuronyltransferase and N-acetylgalactosaminyltransferase activities are observed when chondroitin, chondroitin sulfate polymer, and their corresponding oligosaccharides are used as the acceptor substrates, reaction products were shown to have a GlcAbeta(13)GalNAc linkage and a GalNAbeta(14)GlcA linkage in the nonreducing terminus of chondroitin | Homo sapiens | ? | - |
? | |
| UDP-glucuronic acid + chondroitin sulfate CS-11 | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chondroitin sulfate synthase-3 | - |
Homo sapiens |
| CSS3 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
Mes buffer, reaction with chondroitin sulfate C11 and UDP-glucuronic acid | Homo sapiens |
| 6.2 | - |
Mes buffer, reaction with chondroitin sulfate C10 and UDP-GalNAc | Homo sapiens |
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