Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.174 extracted from

  • Izumikawa, T.; Sato, B.; Mikami, T.; Tamura, J.; Igarashi, M.; Kitagawa, H.
    GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) is the preferred substrate for chondroitin N-acetylgalactosaminyltransferase-1 (2015), J. Biol. Chem., 290, 5438-5448.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.174

Cloned(Commentary)

Cloned (Comment) Organism
gene CSGALNACT1, quantitative real-time RT-PCR enzyme expression analysis Mus musculus
gene CSGALNACT2, quantitative real-time RT-PCR enzyme expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme knockout mutant ChGn-1-/- Mus musculus
additional information construction of enzyme knockout mutant ChGn-2-/- Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q8BJQ9 gene csgalnact1
-
Mus musculus Q8C1F4 gene csgalnact2
-
Mus musculus C57BL/6 Q8BJQ9 gene csgalnact1
-
Mus musculus C57BL/6 Q8C1F4 gene csgalnact2
-

Source Tissue

Source Tissue Comment Organism Textmining
cartilage growth plate cartilage Mus musculus
-
embryo
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) Mus musculus ?
-
 ?
additional information no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) Mus musculus C57BL/6 ?
-
 ?
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) a phosphorylated tetrasaccharide linkage structure in ChGn-1-/- growth plate cartilage but not in ChGn-2-/- or wild-type growth plate cartilage, preferred substrate of ChGn-1 Mus musculus UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)
-
 ?
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) a phosphorylated tetrasaccharide linkage structure in ChGn-1-/- growth plate cartilage but not in ChGn-2-/- or wild-type growth plate cartilage, preferred substrate of ChGn-1 Mus musculus C57BL/6 UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)
-
 ?
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin
-
 Mus musculus UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin
-
 ?
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin
-
 Mus musculus C57BL/6 UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin
-
 ?

Synonyms

Synonyms Comment Organism
ChGn-1
-
 Mus musculus
ChGn-2
-
 Mus musculus
chondroitin N-acetylgalactosaminyltransferase-1
-
 Mus musculus
chondroitin N-acetylgalactosaminyltransferase-2
-
 Mus musculus
GalNAcT-I
-
 Mus musculus
GalNAcT-II
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Mus musculus

General Information

General Information Comment Organism
malfunction deficiency in chondroitin N-acetylgalactosaminyltransferase-1 reduces the number of chondroitin sulfate chains, leading to skeletal dysplasias in mice. Accumulation of phosphorylated pentasaccharide and tetrasaccharide linkages in ChGn-1-/- growth plate cartilage Mus musculus
additional information proportion of linkage region saccharides from wild-type, ChGn-1-/-, or ChGn-2-/- cartilage, overview Mus musculus
physiological function chondroitin N-acetylgalactosaminyltransferase-1 regulates the number of chondroitin sulfate chains for normal cartilage development. ChGn-1-mediated addition of N-acetylgalactosamine is accompanied by rapid 2-phosphoxylose phosphatase XYLP-dependent dephosphorylation during formation of the CS linkage region. ChGn-1 and XYLP cooperatively regulate the number of chondroitin sulfate chains in growth plate cartilage Mus musculus