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Literature summary for 2.4.1.174 extracted from

  • Gotoh, M.; Sato, T.; Akashima, T.; Iwasaki, H.; Kameyama, A.; Mochizuki, H.; Yada, T.; Inaba, N.; Zhang, Y.; Kikuchi, N.; Kwon, Y.D.; Togayachi, A.; Kudo, T.; Nishihara, S.; Watanabe, H.; Kimata, K.; Narimatsu, H.
    Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis (2002), J. Biol. Chem., 277, 38189-38196.
    View publication on PubMed
Search for more literature for 2.4.1.174

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression of a truncated form, consisting of the putative catalytic domain, in Spodoptera frugiperda Sf21 insect cells as a soluble FLAG-tagged protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides Homo sapiens involved in chondroitin initiation and elongation UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides Homo sapiens involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core. UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TDX6 chondroitin sulfate N-acetylgalactosaminyltransferase
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland low content Homo sapiens
-
bone marrow
-
 Homo sapiens
-
brain
-
 Homo sapiens
-
cerebral cortex
-
 Homo sapiens
-
colon low content Homo sapiens
-
G-361 cell melanoma cell line Homo sapiens
-
kidney very low content Homo sapiens
-
leukocyte low content Homo sapiens
-
liver very low content Homo sapiens
-
lung low content Homo sapiens
-
ovary
-
 Homo sapiens
-
pancreas
-
 Homo sapiens
-
placenta very high content Homo sapiens
-
small intestine high content Homo sapiens
-
spleen
-
 Homo sapiens
-
SW-1736 cell thyroid cancer cell line Homo sapiens
-
testis low content Homo sapiens
-
thymus low content Homo sapiens
-
thyroid gland very high content Homo sapiens
-
U266 cell myeloma cell line Homo sapiens
-
uterus
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl i.e. linkage tetrasaccharide, synthetic substrate Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
-
 ?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl best acceptor substrate together with chondroitin polysaccharide Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
-
 ?
UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl transfers GalNAc to GlcA at the non-reducing terminus of the linkage tetrasacharide Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides
-
 Homo sapiens UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides involved in chondroitin initiation and elongation Homo sapiens UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core. Homo sapiens UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme has also N-acetylgalactosaminyltransferase II activity, EC 2.4.1.175, substrates chondroitin oligo- and polysaccharides Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Homo sapiens