Cloned (Comment) | Organism |
---|---|
gene SGT1, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 | Solanum lycopersicum |
gene SGT2, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 | Solanum lycopersicum |
gene SGT3, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 | Solanum lycopersicum |
gene SGT4, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1 | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Solanum lycopersicum | 5829 | - |
additional information | SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview | Solanum lycopersicum | - |
- |
plasma membrane | - |
Solanum lycopersicum | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + a sterol | Solanum lycopersicum | - |
UDP + a sterol 3-beta-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | A0A2D1N4Z8 | cv. Micro-Tom | - |
Solanum lycopersicum | K4BS77 | cv. Micro-Tom | - |
Solanum lycopersicum | K4BTE6 | cv. Micro-Tom | - |
Solanum lycopersicum | K4C3H8 | cv. Micro-Tom | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Solanum lycopersicum | - |
fruit | - |
Solanum lycopersicum | - |
fruit | expression of SlSGT3 is clearly different from other isozymes since its mRNA levels are low in developing fruits (small green and mature green stages) but increase sharply when fruits start to ripe and remain at similar high levels until the red mature stage | Solanum lycopersicum | - |
fruit | in small green, mature green, and mature red fruits. SlSGT1 mRNA levels decrease when fruits start to ripe (breaker and orange stages) but increase again in red ripe fruits | Solanum lycopersicum | - |
fruit | in small green, mature green, and mature red fruits. SlSGT2 mRNA levels decrease when fruits start to ripe (breaker and orange stages) but increase again in red ripe fruits | Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
additional information | the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening | Solanum lycopersicum | - |
additional information | the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits | Solanum lycopersicum | - |
additional information | the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed. | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
seedling | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose | Solanum lycopersicum | ? | - |
- |
|
UDP-glucose + a sterol | - |
Solanum lycopersicum | UDP + a sterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + beta-sitosterol | - |
Solanum lycopersicum | UDP + beta-sitosterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + brassicasterol | - |
Solanum lycopersicum | UDP + brassicasterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + campesterol | - |
Solanum lycopersicum | UDP + campesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + stigmasterol | - |
Solanum lycopersicum | UDP + stigmasterol 3-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SGT1 | - |
Solanum lycopersicum |
SGT2 | - |
Solanum lycopersicum |
SGT3 | - |
Solanum lycopersicum |
SGT4 | - |
Solanum lycopersicum |
SlSGT1 | - |
Solanum lycopersicum |
SlSGT2 | - |
Solanum lycopersicum |
SlSGT3 | - |
Solanum lycopersicum |
SlSGT4 | - |
Solanum lycopersicum |
Solyc04g051150.2.1 | locus name | Solanum lycopersicum |
Solyc04g071540.2.1 | locus name | Solanum lycopersicum |
Solyc06g007980.2.1 | locus name | Solanum lycopersicum |
Solyc09g061860.2.1 | locus name | Solanum lycopersicum |
UDP-glucose sterol glycosyltransferase | - |
Solanum lycopersicum |
UDP-glucose:sterol glucosyltransferase | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Solanum lycopersicum |
Organism | Comment | Expression |
---|---|---|
Solanum lycopersicum | transcriptional profiling of SlSGT gene expression in response to stress, overview | additional information |
Solanum lycopersicum | transcriptional profiling of SlSGT gene expression in response to stress, overview. SlSGT1 expression remains almost unaltered under the tested stress conditions | additional information |
Solanum lycopersicum | transcriptional profiling of SlSGT gene expression in response to stress, overview. SlSGT3 expression remains almost unaltered under the tested stress conditions | additional information |
Solanum lycopersicum | SlSGT2 expression increases markedly in response to osmotic, salt, and cold stress, as well as upon treatment with abscisic acid and methyl jasmonate | up |
Solanum lycopersicum | SlSGT4 expression increases markedly in response to osmotic, salt, and cold stress, as well as upon treatment with abscisic acid and methyl jasmonate | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the UDP-glucose sterol glycosyltransferases family of developmental and stress regulated genes that encode cytosolic and membrane-associated forms of the enzyme. Identification and functional characterization of the four members, SlSGT1-4, of the tomato cv. Micro-Tom | Solanum lycopersicum |
physiological function | sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Solanum contain very high levels of glycosylated sterols, which in the case of tomato may account for more than 85% of the total sterol content. Tomato SGT isozymes show the ability to glycosylate different sterol species including cholesterol, brassicasterol, campesterol, stigmasterol, and beta-sitosterol, which is consistent with the occurrence in their primary structure of the putative steroid-binding domain found in steroid UDP-glucuronosyltransferases and the UDP-sugar binding domain characteristic for a superfamily of nucleoside diphosphosugar glycosyltransferases. The SlSGT isozyme are differentially regulated in response to biotic and abiotic stress conditions. Changes in the relative proportions of sterols alter membrane fluidity and permeability and hence regulate different membrane functions such as simple and carrier-mediated diffusion, active transport across the membrane, and the activity of membrane-associated proteins. Tomato SGT isozymes play overlapping but not completely redundant biological functions involved in mediating developmental and stress responses | Solanum lycopersicum |
physiological function | sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Solanum contain very high levels of glycosylated sterols, which in the case of tomato may account for more than 85% of the total sterol content. Tomato SGT isozymes show the ability to glycosylate different sterol species including cholesterol, brassicasterol, campesterol, stigmasterol, and beta-sitosterol, which is consistent with the occurrence in their primary structure of the putative steroid-binding domain found in steroid UDP-glucuronosyltransferases and the UDP-sugar binding domain characteristic for a superfamily of nucleoside diphosphosugar glycosyltransferases. The SlSGT isozyme are differentially regulated in response to biotic and abiotic stress conditions. Tomato SGT isozymes play overlapping but not completely redundant biological functions involved in mediating developmental and stress responses. Changes in the relative proportions of sterols alter membrane fluidity and permeability and hence regulate different membrane functions such as simple and carrier-mediated diffusion, active transport across the membrane, and the activity of membrane-associated proteins | Solanum lycopersicum |