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Literature summary for 2.4.1.173 extracted from

  • Warnecke, D.; Erdmann, R.; Fahl, A.; Hube, B.; Muller, F.; Zank, T.; Zahringer, U.; Heinz, E.
    Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum (1999), J. Biol. Chem., 274, 13048-13059.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Candida albicans
expression in Escherichia coli Dictyostelium discoideum
expression in Escherichia coli Komagataella pastoris
expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information null mutant by deletion of ugt52 gene with no enzyme activity Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
114000
-
cloned, calculated molecular mass Dictyostelium discoideum
136000
-
cloned, calculated molecular mass Komagataella pastoris
136000
-
cloned, calculated molecular mass Saccharomyces cerevisiae
171000
-
cloned, calculated molecular mass Candida albicans

Organism

Organism UniProt Comment Textmining
Avena sativa O22678 ugt80A1
-
Avena sativa ugt80A1 O22678 ugt80A1
-
Candida albicans Q5A950 ugt51c1
-
Dictyostelium discoideum Q54IL5 ugt52
-
Dictyostelium discoideum ugt52 Q54IL5 ugt52
-
Komagataella pastoris
-
-
-
Komagataella pastoris Q9Y751 ugt51b1
-
Komagataella pastoris GS115 Q9Y751 ugt51b1
-
Saccharomyces cerevisiae Q06321 ugt51=ylr189C
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Dictyostelium discoideum ?
-
?
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Komagataella pastoris ?
-
?
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Saccharomyces cerevisiae ?
-
?
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Avena sativa ?
-
?
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Dictyostelium discoideum ugt52 ?
-
?
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Komagataella pastoris GS115 ?
-
?
additional information UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor Avena sativa ugt80A1 ?
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Dictyostelium discoideum UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Komagataella pastoris UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Saccharomyces cerevisiae UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Avena sativa UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Dictyostelium discoideum ugt52 UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Komagataella pastoris GS115 UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + cholesterol cloned enzyme glucosylates cholesterol Avena sativa ugt80A1 UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Dictyostelium discoideum UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Komagataella pastoris UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Saccharomyces cerevisiae UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Avena sativa UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Dictyostelium discoideum ugt52 UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Komagataella pastoris GS115 UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + ergosterol cloned enzyme glucosylates ergosterol Avena sativa ugt80A1 UDP + ergosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol
-
Candida albicans UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Dictyostelium discoideum UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Komagataella pastoris UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Saccharomyces cerevisiae UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Avena sativa UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Dictyostelium discoideum ugt52 UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Komagataella pastoris GS115 UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + sitosterol cloned enzyme glucosylates sitosterol Avena sativa ugt80A1 UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Dictyostelium discoideum UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Komagataella pastoris UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Saccharomyces cerevisiae UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Avena sativa UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Dictyostelium discoideum ugt52 UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Komagataella pastoris GS115 UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol cloned enzyme glucosylates stigmasterol Avena sativa ugt80A1 UDP + stigmasterol 3-beta-D-glucoside
-
?
UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Dictyostelium discoideum UDP + tomatidine 3-beta-glucoside
-
?
UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Komagataella pastoris UDP + tomatidine 3-beta-glucoside
-
?
UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Saccharomyces cerevisiae UDP + tomatidine 3-beta-glucoside
-
?
UDP-glucose + tomatidine cloned enzyme glucosylates tomatidine Avena sativa UDP + tomatidine 3-beta-glucoside
-
?

Synonyms

Synonyms Comment Organism
Ugt51
-
Komagataella pastoris

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Candida albicans
7.5
-
Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Dictyostelium discoideum
7.5
-
Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Komagataella pastoris
7.5
-
Tris-HCl 100 mM, dithiothreitol 5 mM, pefabloc 200 uM Saccharomyces cerevisiae