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Literature summary for 2.4.1.173 extracted from

  • Ullmann, P.; Bouvier-Nave, P.; Benveniste, P.
    Regulation by phospholipids and kinetic studies of plant membrane-bound UDP-glucose sterol beta-D-glucosyl transferase (1987), Plant Physiol., 85, 51-55.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine increases Vmax Zea mays
phosphatidylglycerol increases Vmax Zea mays
Phospholipids stimulated by negatively charged phospholipids Zea mays
Phospholipids phosphatidylcholine or phosphatidylglycerol increase Vmax Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 UDP-glucose slightly lowered by phosphatidylcholine and phosphatidylglycerol Zea mays
0.2
-
 sitosterol strongly lowered by phosphatidylcholine and phosphatidylglycerol Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Zea mays
-
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation and DEAE-cellulose column Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
coleoptile
-
 Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + sitosterol
-
 Zea mays UDP + sitosterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Zea mays

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 Tris-HCl 50 mM, 1 mM 2-mercaptoethanol, emulphogene BC720 0.36% Zea mays