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Literature summary for 2.4.1.170 extracted from
- Molecular cloning and characterization of an isoflavone 7-O-glucosyltransferase from Pueraria lobata (2014), Plant Cell Rep., 33, 1173-1185.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PlUGT1 or UGT88E12, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli and in Saccharomyces cerevisiae strain WAT11 | Pueraria montana var. lobata |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Saccharomyces cerevisiae cells expressing PlUGT1 are not able to glycosylate daidzin and puerarin | Pueraria montana var. lobata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0204 | - |
genistein | pH 8.0, 30°C | Pueraria montana var. lobata |  |
| 0.0299 | - |
daidzein | pH 8.0, 30°C | Pueraria montana var. lobata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + daidzein | Pueraria montana var. lobata | - |
UDP + daidzein 7-O-beta-D-glucoside | i.e. daidzin | ? | |
| UDP-glucose + formononetin | Pueraria montana var. lobata | - |
UDP + formononetin 7-O-beta-D-glucoside | i.e. ononin | ? | |
| UDP-glucose + genistein | Pueraria montana var. lobata | - |
UDP + genistein 7-O-beta-D-glucoside | i.e. genistin | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pueraria montana var. lobata | - |
collected from Langxi County, Anhui Province, China, gene PlUGT1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Pueraria montana var. lobata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Pueraria montana var. lobata | - |
| additional information | transcript abundance of gee PlUGT1 is correlated with the accumulation pattern of isoflavone glycosides such as daidzin in Pueraria lobata plants or in cell suspension culture | Pueraria montana var. lobata | - |
| root | high enzyme expression level | Pueraria montana var. lobata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is relatively specific for isoflavone aglycones, while flavonoid substrates are poorly accepted. No activity with cyanidin, luteolin, kaempferol, and quercetin, naringenin and apigenin are poor substrates, substrate specificity, overview | Pueraria montana var. lobata | ? | - |
? | |
| UDP-alpha-D-glucose + isoliquiritigenin | - |
Pueraria montana var. lobata | UDP + isoliquiritigenin 7-O-beta-D-glucoside | - |
? | |
| UDP-glucose + daidzein | - |
Pueraria montana var. lobata | UDP + daidzein 7-O-beta-D-glucoside | i.e. daidzin | ? | |
| UDP-glucose + daidzein | high activity | Pueraria montana var. lobata | UDP + daidzein 7-O-beta-D-glucoside | i.e. daidzin | ? | |
| UDP-glucose + formononetin | - |
Pueraria montana var. lobata | UDP + formononetin 7-O-beta-D-glucoside | i.e. ononin | ? | |
| UDP-glucose + genistein | - |
Pueraria montana var. lobata | UDP + genistein 7-O-beta-D-glucoside | i.e. genistin | ? | |
| UDP-glucose + genistein | best substrate | Pueraria montana var. lobata | UDP + genistein 7-O-beta-D-glucoside | i.e. genistin | ? | |
| UDP-glucose + liquiritigenin | very low activity | Pueraria montana var. lobata | UDP + liquiritigenin 7-O-beta-D-glucoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PlUGT1 | - |
Pueraria montana var. lobata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pueraria montana var. lobata |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
genistein | pH 8.0, 30°C | Pueraria montana var. lobata | |
| 1.1 | - |
daidzein | pH 8.0, 30°C | Pueraria montana var. lobata | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pueraria montana var. lobata |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pueraria montana var. lobata | the enzyme is strongly induced by methyl jasmonate signal in cell suspension culture | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 37 | - |
daidzein | pH 8.0, 30°C | Pueraria montana var. lobata | |
| 54 | - |
genistein | pH 8.0, 30°C | Pueraria montana var. lobata | |
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