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Literature summary for 2.4.1.170 extracted from

  • Nagashima, S.; Inagaki, R.; Kubo, A.; Hirotani, M.; Yoshikawa, T.
    cDNA cloning and expression of isoflavonoid-specific glucosyltransferase from Glycyrrhiza echinata cell-suspension cultures (2004), Planta, 218, 456-459.
    View publication on PubMed
Search for more literature for 2.4.1.170

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is stress-inducible, transient induction by cell suspension culture treatment with yeast extract Glycyrrhiza echinata

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Glycyrrhiza echinata

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is stress-inducible, no inhibition by baicalein, kaempferol, naringenin, apigenin, quercetin, scopoletin, ferulic acid, and salicylic acid Glycyrrhiza echinata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48700
-
 x * 48700, about, DNA sequence calculation Glycyrrhiza echinata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycyrrhiza echinata the enzyme might play a role in defense against external stresses such as UV radiation and pathogen attack ?
-
 ?

Organism

Organism UniProt Comment Textmining
Glycyrrhiza echinata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography Glycyrrhiza echinata

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture transient induction by cell suspension culture treatment with yeast extract Glycyrrhiza echinata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme might play a role in defense against external stresses such as UV radiation and pathogen attack Glycyrrhiza echinata ?
-
 ?
additional information substrate specificity, the flavonoids kaempferol, apigenin, and quercetin are poor substrates Glycyrrhiza echinata ?
-
 ?
UDP-glucose + baicalein very low activity Glycyrrhiza echinata UDP + baicalein 7-O-beta-D-glucoside
-
 ?
UDP-glucose + daidzein
-
 Glycyrrhiza echinata UDP + daidzein 7-O-beta-D-glucoside
-
 ?
UDP-glucose + ferulic acid low activity Glycyrrhiza echinata UDP + 1-O-feruloyl-beta-D-glucose
-
 ?
UDP-glucose + formononetin best substrate Glycyrrhiza echinata UDP + formononetin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + naringenin low activity Glycyrrhiza echinata UDP + naringenin 7-O-beta-D-glucoside
-
 ?
UDP-glucose + salicylic acid very low activity Glycyrrhiza echinata UDP + salicylic acid 7-O-beta-D-glucoside
-
 ?
UDP-glucose + scopoletin low activity Glycyrrhiza echinata UDP + scopoletin 7-O-beta-D-glucoside
-
 ?

Subunits

Subunits Comment Organism
? x * 48700, about, DNA sequence calculation Glycyrrhiza echinata

Synonyms

Synonyms Comment Organism
additional information the enzyme belongs to the family of UDP-glycosyltransferases Glycyrrhiza echinata
UDP-glucose: formononetin 7-O-glucosyltransferase
-
 Glycyrrhiza echinata
UGT73F1
-
 Glycyrrhiza echinata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Glycyrrhiza echinata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Glycyrrhiza echinata

pI Value

Organism Comment pI Value Maximum pI Value
Glycyrrhiza echinata sequence calculation
-
 5.6