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Literature summary for 2.4.1.17 extracted from

  • Kurkela, M.; Morsky, S.; Hirvonen, J.; Kostiainen, R.; Finel, M.
    An active and water-soluble truncation mutant of the human UDP-glucuronosyltransferase 1A9 (2004), Mol. Pharmacol., 65, 826-831.
    View publication on PubMed
Search for more literature for 2.4.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme UGT1A9 lacking 45 residues of the C-terminus including the trans-membrane helix, enzyme mutant is His6-tagged at the shortened C-terminus, detergent-free expression as soluble protein in insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information either detergent binding to the transmembrane helix within the full-length isozyme UGT1A9 or removal of this helix affect the interaction of the enzyme with its aglycone substrate in a similar manner Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 inhibition of recombinant soluble isozyme UGT1A9 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60656 isozyme UGT1A9; isozyme UGT1A9
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme UGT1A9 mutant from insect cells by nickel affinity chromatogaphy Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificities of wild-type and recombinant soluble mutant isozyme UGT1A9 Homo sapiens ?
-
 ?
UDP-glucuronate + 1-naphthol
-
 Homo sapiens UDP + 1-naphthol beta-D-glucuronide
-
 ?
UDP-glucuronate + 4-nitrophenol
-
 Homo sapiens UDP + 4-nitrophenol beta-D-glucuronide
-
 ?
UDP-glucuronate + entacapone
-
 Homo sapiens UDP + entacapone beta-D-glucuronide
-
 ?
UDP-glucuronate + scopoletin
-
 Homo sapiens UDP + scopoletin beta-D-glucuronide
-
 ?
UDP-glucuronate + umbelliferone
-
 Homo sapiens UDP + umbelliferone beta-D-glucuronide
-
 ?

Subunits

Subunits Comment Organism
additional information
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
UDP-glucuronosyltransferase
-
 Homo sapiens
UGT
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens